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Novel multi-section flat type photo-bioreactor

A photobioreactor and reactor technology, applied in photobioreactor, bioreactor/fermenter for specific purposes, bioreactor/fermenter combination, etc., can solve the problem that oxygen analysis cannot be carried out effectively, algal culture Efficiency effects, restrictions on the application of built-in light sources, etc., to achieve the effects of high light energy utilization efficiency, easy processing and promotion, and promotion of liquid circulation

Active Publication Date: 2010-02-03
JIAXING ZEYUAN BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the hanging bag system is mainly used for secondary seed cultivation in China, but there are still some problems in the hanging bag system: for example, after the volume of the container is enlarged, the specific surface area of ​​the light is relatively small due to the relatively large diameter of the container, resulting in light-limited cultivation ; The ventilation system is not smooth, resulting in insufficient mixing of the algae liquid in the container, and the ineffective oxygen desorption, etc., which have a great impact on the cultivation efficiency of the algae
The external light source is easy to operate, but the light energy utilization efficiency is low; the built-in light source greatly improves the light utilization rate, but the application of the built-in light source is limited due to the heat generated and the wall attachment of the cells

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Embodiment 1: Adopt the comparison of the present invention and single-section plate photobioreactor culture efficiency

[0086] Under natural light, the cultivation efficiency of the 1000L multi-section-air-lift type-internal light source-flat-panel photobioreactor of the present invention and the 1000L single-section air-lift type flat-panel photobioreactor were compared. The medium used is: NaNO 3 : 90mg / L, KH 2 PO 4 : 5mg / L, FeC 6 h 5 o 7 ·5H 2 O: 0.5mg / L; the culture temperature is 25-30℃; the actual liquid volume of 1000L multi-section plate reactor and 1000L single-section plate reactor is about 800L, and the gas-liquid ratio is 0.1vvm; the algae species used for cultivation are Isoflagellates 3011 (commercially available).

[0087] The seawater used in the test was preliminarily treated by sedimentation, pressurized sand filtration, etc., and then sterilized by oxidation. Before use, add about 1L of commercially available bleach solution (with an availabl...

Embodiment 2

[0089] Embodiment 2: adopt the high-density cultivation of the present invention to widely use Phaeodactylum tricornutum in seawater seedling cultivation

[0090] The medium used is: NaNO 3 : 90mg / L, KH 2 PO 4 : 5mg / L, FeC 6 h 5 o 7 ·5H 2 O: 0.5mg / L; the culture temperature is 20-25°C; the actual liquid volume of a 1000L plate reactor is about 800L. The seawater treatment method used in the test is the same as in Example 1.

[0091] Under the situation of natural light and increasing artificial light source respectively, compared the cultivation efficiency of 1000L plate type reactor of the present invention and 300L cylindrical type reactor, 300L cylindrical type reactor is a kind of standing bag with support on the periphery, is the current domestic A more advanced culture system for industrial bait microalgae culture. During the cultivation process, the average natural light intensity on the surface of various reactors is: about 8000lx on the sunny side and about 35...

Embodiment 3

[0096] Embodiment 3: Isochrysis globosa 3011 widely used in seawater seedling cultivation by high-density culture of the present invention

[0097] The medium used is: NaNO 3 : 90mg / L, KH 2 PO 4 : 5mg / L, FeC 6 h 5 o 7 ·5H 2 O: 0.5mg / L; the culture temperature is 24-32°C; the actual liquid volume of a 1000L plate reactor is about 800L. The seawater treatment method used in the test is the same as in Example 1.

[0098] The cultivation efficiency of the 1000L multi-section-airlift-inner light source-flat plate photobioreactor and the 300L cylindrical type reactor of the present invention were compared under the conditions of natural light and artificial light source respectively. In the artificial light source group, when the daytime (8:00-18:00) eye light intensity is less than 8000lx, artificial light source is added, and the tube-liquid volume ratio is 6 tubes / 300L, of which 1000L flat plate reactor artificial light source The form is an internal light source, and the...

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Abstract

The invention discloses a flat optical biological reactor, which consists of transparent flat box and gas-supplying device, wherein multiple hollow diversion baffle cavities are set in the box, whichcontain one or more artificial light source; the cavity is set on the vertical plane in the reactor with gap among each diversion baffle cavity.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to the field of high-density cultivation of microalgae, and more specifically relates to a novel microalgae cultivation system of a plate type photobioreactor. Background technique [0002] The application of photobioreactors to high-efficiency cultivation of microalgae has been a hot research topic at home and abroad in recent years. At present, the large-scale cultivation of algae is basically open cultivation, which has the disadvantages of easy pollution, difficult control and low efficiency. In order to overcome the above shortcomings, people began to study the application of closed photobioreactors for the cultivation of microalgae, and various closed photobioreactors have been developed. The basic forms of photobioreactors mainly include tubular reactors, column reactors and plate reactors. The basic ideas used in the design of these reactors are two: 1) reduce the optical pat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/00
CPCC12M21/02C12M27/20C12M23/04C12M31/10
Inventor 李元广沈国敏于钢孙新志李兴武万俊芬程鑫张静霞林晨
Owner JIAXING ZEYUAN BIOLOGICAL PROD
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