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Peptide composition

A drug and amino acid technology, applied in the field of peptide application, can solve problems such as low efficacy

Inactive Publication Date: 2009-09-09
PEPSYN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its efficacy is not very high

Method used

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  • Peptide composition
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0262] Example 1 - Preparation of standardized natural products from cheese whey

[0263] This step includes methods for collecting, preparing and storing standardized natural products (SNPs) from cheese whey. This step is often used to prepare SNPs on a small scale, such as for research or improvement purposes. However, according to the prior art, this method can be scaled up for commercial production as needed.

[0264] Collection and storage of cheese whey

[0265] Approximately 401 fresh pure cheese whey was obtained from DewLay Cheese Factory (Garstang, Lancashire). The whey is collected in clean containers and sent immediately to the Pepsyn Central Manufacturing Plant (Liverpool).

[0266] The whey can be refrigerated for later processing, or it can be frozen at -20°C in a shallow container at 21 until use.

[0267] Thawing of Cheese Whey

[0268] Place 21 whey cubes in a plastic bag and submerge in hot running water to thaw the whey. Thawing is completed within 10...

Embodiment 2

[0282] Example 2 - Demonstration that SNPs increase collagen synthesis in fibroblasts

[0283] Rama27 rat cells were grown to confluence and their rate of collagen synthesis was measured by the method of M.J. Warburton, S.A. Ferns and P.S. Rudland, Experimental Cell Research, 137, 373-380 (1982). Collagen synthesis rates calculated by incorporating [3H]proline into hydroxyproline are listed in Table 2 below:

[0284] Table 2. Rate of Collagen Synthesis

[0285]

[0286] Addition of 0.6 mg / ml of SNP increased collagen secretion approximately 12-fold—from 54 cpm to 663 cpm. This also approximately tripled the ratio of secreted synthetic collagen to collagen contained in the cells—from 54:53 (1:1) to 663:321 (2:1).

Embodiment 3

[0287] Example 3 - Demonstration of the effect of SNPs on keratinocyte growth

[0288] Human keratinocytes (HatKat) were grown in Keratinocyte Growth Medium (TCS Cellworks Ltd.) until 20% confluent. Then, 0.5% fetal calf serum (FCS) was added to the same medium, and the keratinocytes were grown for 3 days. The counter counts the cells. The resulting cell numbers are listed in Table 3 below.

[0289] Table 3. Cell Numbers

[0290]

[0291] This indicated that the presence of the SNP increased keratinocyte growth approximately 3-fold—from 23,777-68,719. In contrast, the growth obtained with 10 ng / ml EGF was relatively moderate.

[0292] These results clearly demonstrate the collagen-producing and growth-promoting activity of the peptides used in the present invention.

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Abstract

Provided is use of a peptide, or a derivative of a peptide, in the manufacture of a medicament effective in alleviating or preventing periodontal disease, wherein the peptide comprises an amino acid sequence present in an alpha-S2 casein precursor, said sequence comprising 3 or more amino acids, and not comprising at its N-terminus the N-terminal amino acids of the full alpha-S2 casein precursor. The peptide may alternatively be any peptide having an alpha-S2 casein fragment activity. Further provided is use of a peptide, or a derivative of a peptide, in the manufacture of a medicament effective in alleviating or preventing an effect of aging in skin, wherein the peptide comprises an amino acid sequence present in a n alpha-S2 casein precursor, said sequence comprising 3 or more amino acids, and not comprising at its N-terminus the N-terminal amino acid of the full alpha-S2 casein precursor. The peptide may alternatively be any peptide having an alpha-S2 casein fragment activity.

Description

technical field [0001] The present invention relates to proteins, peptides (usually polypeptides), peptide derivatives or peptide fragments which can be used to reduce or prevent the effects of aging, especially skin aging effects. It can be used in therapeutic or cosmetic methods. The present invention also relates to the same peptides, polypeptides, peptide derivatives or peptide fragments which are useful in the prophylaxis or treatment of periodontal disease (gum disease). It can be used in medical treatment methods if desired. In particular, the present invention relates to the use of peptides containing the amino acid sequence of alpha-S2 casein precursor. Background technique [0002] Milk has been known for many years to contain, in addition to its nutrients, activities that promote cell growth. In this regard, it has been demonstrated that epidermal growth factor (EGF). [0003] EGF present in rat milk is important for normal development of pups (Oka et al., 19...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61K8/64C07K14/47A61P17/00A61Q19/08A61K8/72A61K8/00A61K38/00A61P1/02A61P43/00A61Q11/00A61Q19/00C07K7/04C07K7/08
CPCA61K8/64C07K14/4732A61Q11/00A61K38/00A61Q19/08A61P1/02A61P17/00A61P43/00
Inventor J·A·史密斯
Owner PEPSYN
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