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Method for detecting chemical luminous analysis of chloro-phenol

A technology of chemiluminescence and analysis method, which is applied in the field of chemiluminescence reaction of determination materials, and can solve the problems of low recovery rate, low determination sensitivity, and expensive instruments.

Inactive Publication Date: 2009-06-17
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sensitivity of spectrophotometry is low, and different CPs need different processing methods, which are detected at different wavelengths, and the operation is cumbersome; GC and HPLC methods cannot directly measure CPs, requiring derivatization treatment, cumbersome operation, and poor reproducibility , the recovery rate is low, and the instrument is expensive and the use is limited [1]

Method used

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  • Method for detecting chemical luminous analysis of chloro-phenol
  • Method for detecting chemical luminous analysis of chloro-phenol

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0040] Example 1 (determination of 2,4-dichlorophenol)

[0041] 1. Configure reagents

[0042] Sample stock solution: Accurately weigh 0.143g 2,4-dichlorophenol (analytical pure), use 1×10 -3 mol / L NaOH solution is dissolved and transferred to a 250mL brown volumetric flask, stored in a refrigerator (4°C) away from light, and diluted step by step when used; 5×10 -4 mol / L fluorescein stock solution: accurately weigh a certain amount of fluorescein, and use 1×10 -3 mol / L NaOH is dissolved and diluted step by step when used; 1.0×10 -3 mol / L N-bromosuccinimide (NBS) stock solution: 1.16×10 -1 mol / L Triton X-100 solution and 0.05mol / L borax solution, 0.25mol / L NaH 2 PO 4 are configured in the usual way. The reagents used were of analytical grade unless otherwise specified, and the water was high-purity deionized water.

[0043] 2. Experimental method

[0044] Take a certain amount of chlorophenol sample, add 1.0ml5.0×10 -4 mol / L fluorescein, 5.0ml0.25mol / LNaH 2 PO 4 Buff...

example 2

[0054] Example 2 (2, the determination of 4-chlorophenol)

[0055] 1. Configure reagents

[0056] Sample stock solution: Accurately weigh 0.143g 2,4-dichlorophenol (analytical pure), use 1×10 -3 mol / L NaOH solution is dissolved and transferred to a 250mL brown volumetric flask, stored in a refrigerator (4°C) away from light, and diluted step by step when used; 5×10 -4 mol / L methylene blue stock solution: accurately weigh a certain amount of methylene blue, and use 1×10 -3 mol / L NaOH is dissolved and diluted step by step when used; 1.0×10 -3 mol / L N-bromosuccinimide (NBS) stock solution, 1.16×10 -1 mol / L Triton X-100 solution and 0.05mol / L borax solution, 0.25mol / L NaH 2 PO 4 are configured in the usual way. The reagents used were of analytical grade unless otherwise specified, and the water was high-purity deionized water.

[0057] 2. Experimental method

[0058] Take a certain amount of chlorophenol sample, add 2.5ml5.0×10 -4 mol / L methylene blue, 5.0ml0.25mol / L NaH ...

example 3

[0068] Example 3 (2,4, the determination of 6-trichlorophenol)

[0069] 1. Configure reagents

[0070] Sample stock solution: Accurately weigh 0.173g 2,4,6-trichlorophenol (analytical pure), use 1×10 -3 mol / L NaOH solution is dissolved and transferred to a 250mL brown volumetric flask, stored in a refrigerator (4°C) away from light, and diluted step by step when used; 5×10 -4 mol / L fluorescein stock solution: accurately weigh a certain amount of fluorescein, and use 1×10 -3 mol / L NaOH is dissolved and diluted step by step when used; 1.0×10 -3 mol / L dibromohydantoin stock solution, 1.16×10 -1 mol / L Triton X-100 solution and 0.05mol / L borax solution, 0.25mol / L NaH2 PO 4 are configured in the usual way. The reagents used were of analytical grade unless otherwise specified, and the water was high-purity deionized water.

[0071] 2. Experimental method

[0072] Take a certain amount of chlorophenol sample, add 1.0ml 5.0×10 -4 mol / L fluorescein, 5.0ml0.25mol / L NaH 2 PO 4 B...

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Abstract

The new method for measuring chlorophenol in high susceptiveness and selectivity is built based on chemiluminescent phenomena generated from reaction between photolyzed chlorophenol and oxidant when fluorescent agent exists. Triton X-100 as sensitization agent can enhance luminous intensity of the system effectively. The method gives out favorable luminescence response for multiform chlorophenol including 4-chlorophenol, 2-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, and pentachloro phenol. Detection limit reaches to 5.0*10-8mol / L. Relative standard deviation of measuring 2,4-dichlorophenol 2,4,6-trichlorophenol in 3.6*10-5mol / L for 7 times is 2.2%. Relative standard deviation of measuring 2,4,6-trichlorophenol in 3.6*10-5mol / L for 7 times is 1.9%. The method carries out experiments for selected multiple possible interfering materials. Results of the experiments indicate that it is lesser interference between each chlorophenol under different pH values.

Description

technical field [0001] The invention belongs to a method for testing or analyzing materials by means of measuring chemical properties of substances, and in particular relates to measuring chemiluminescent reactions of materials. Background technique [0002] When phenolic-contaminated river water is disinfected by chlorine, the phenolic substances in the water will be oxidized to form chlorophenolic compounds (CPs). Long-term drinking of chlorophenol-containing water can cause dizziness, rash, skin itching, anemia and various neurological symptoms. Systemic diseases, acute poisoning symptoms will occur if excessive intake [1] . The National Standard of the People's Republic of China (1999) "Drinking Water Quality Index" stipulates that the contents of 2,4-dichlorophenol, 2,4,6-trichlorophenol and pentachlorophenol are respectively 1 μg / L, 10 μg / L and 0.02mg / L. The People's Republic of China National Standard Surface Water Environmental Quality Standard stipulates that the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76G01N1/38
Inventor 宋启军张俊丽
Owner JIANGNAN UNIV
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