Acoustic fusion of aquatic animal tissue cells with biological agents

a technology of aquatic animal tissue cells and biological agents, applied in biochemistry apparatus and processes, ohmic-resistance heating, enzymes, etc., can solve the problems of high voltage disruption of tissue cells and reduce processing efficiency

Inactive Publication Date: 2005-06-07
THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE NAVY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, infusion of animal tissue cells with biological agents such as tagging compounds was heretofore performed by electroporation subject to various disadvantages such as tissue cell disruption by high voltage and reduced processing efficiency because of limits imposed on the number of tissue cells capable of being simultaneously processed.

Method used

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  • Acoustic fusion of aquatic animal tissue cells with biological agents
  • Acoustic fusion of aquatic animal tissue cells with biological agents
  • Acoustic fusion of aquatic animal tissue cells with biological agents

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Embodiment Construction

[0008]Referring now to the drawing in detail, FIG. 1 diagrams the aquatic animal treatment method of the present invention, generally referred to by reference numeral 10. Pursuant to such method, the tissue cells 12 of aquatic animals to be treated such as migratory fish including rainbow trout as denoted in FIG. 1, are to be fused with a biological agent 14 by insonification which includes perforation and fusion performed within an underwater zone 16 during periods of limited duration so as to accommodate treatment of large populations of the aquatic animals. The agent 14 according to one embodiment is of a commonly used type from a group of tagging compounds including calcein (Fluorexine) and oxytetracyline hydrochloride (Terramycin) forming a solution with the water in the treatment zone 16 from which it is transferred to tissue cells such as the larvae and / or eggs of the fish being tagged. After completion of such treatment the tissue cells are removed from the treatment zone 16...

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Abstract

Biological agents in solution within an underwater treatment zone are transferred by infusion to living cells under an acoustic energy field of limited duration and certain accompanying water flow and temperature conditions to maximize processing efficiency.

Description

[0001]The present invention relates in general to the perforation of living cells during delivery thereto of biological agents for various purposes including tagging and DNA infusion.BACKGROUND OF THE INVENTION[0002]A process for transfer of chemical agents within some surrounding medium through living tissue referred to as poration, is well known in the art as disclosed for example in U.S. Pat. No. 5,128,257 to Baer. Generally, such process involves generation of electrical energy forming an electrical field between electrodes within which electroporation occurs. The generation of an ultra-sound acoustic field within which different chemical compounds are administered by delivery to the tissue cells of aquatic animals or their eggs for fish farm treatment purposes, is disclosed in U.S. Pat. No. 5,076,208 to Zohar et al. However, infusion of animal tissue cells with biological agents such as tagging compounds was heretofore performed by electroporation subject to various disadvantag...

Claims

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Application Information

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IPC IPC(8): C12N13/00C12N15/85C12N15/87
CPCC12N13/00A01K2217/05A01K2227/40C12N15/8509C12N15/87
Inventor CLARK, JOSEPH A.YOUNG, JANE A.
Owner THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE NAVY
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