Peronospora resistance in spinacia oleracea

Abstract

The present invention relates to an allele designated alpha-WOLF 24 which confers resistance to at least one Peronospora farinosa f. sp. spinacea race, wherein the protein encoded by said allele is a CC-NBS-LRR protein that comprises in its amino acid sequence: a) the motif “MAEIGYSVC” SEQ ID NO: 1 at its N-terminus; and b) the motif “KWMCLR” SEQ ID NO: 2; and wherein the LRR domain of the protein has in order of increased preference at least 95%, 96%, 97%, 98%, 98.2%, 98.5%, 98.8%, 99%, 100% sequence similarity to SEQ ID NO: 10. The allele when present in a spinach plant confers complete resistance to at least Peronospora farinosa f. sp. spinacea race Pfs:1, Pfs:2, Pfs:5, Pfs:6, Pfs:7, Pfs:9, Pfs:11, Pfs:13, Pfs:15 and Pfs:17, and does not confer resistance to downy mildew race Pfs:16.

Description

RELATED APPLICATIONS AND INCORPORATION BY REFERENCE[0001]This application claims priority to international patent application Serial No. PCT / EP2020 / 056739 filed 12 Mar. 2020.[0002]The foregoing application, and all documents cited therein or during their prosecution (“appln cited documents”) and all documents cited or referenced in the appln cited documents, and all documents cited or referenced herein (“herein cited documents”), and all documents cited or referenced in herein cited documents, together with any manufacturer's instructions, descriptions, product specifications, and product sheets for any products mentioned herein or in any document incorporated by reference herein, are hereby incorporated herein by reference, and may be employed in the practice of the invention. More specifically, all referenced documents are incorporated by reference to the same extent as if each individual document was specifically and individually indicated to be incorporated by reference.FIELD OF...

AI Technical Summary

Benefits of technology

The invention is about a new resistance allele that provides resistance to a newly emerged downy mildew isolate in spinach plants. The new allele is called alpha-WOLF 24 and was found through molecular biological tools. The invention aims to provide a new resistance profile to pathogenic races of Peronospora farinosa f.sp. spinaciae. The patent does not cover any previously known products, processes, or methods. The invention also includes a deposit of seeds that carry the alpha-WOLF 24 allele withNCIMB Ltd.

Problems solved by technology

spinaciae) is a major threat for spinach growers because it directly affects the harvested leaves.

Infection makes the leaves unsuitable for sale and consumption, as it manifests itself phenotypically as yellow lesions on the older leaves, and on the abaxial leaf surface a greyish fungal growth can be observed.

Especially the latest identified Peronospora races can break the resistance of many spinach varieties that are currently used commercially worldwide, and they thus pose a serious threat to the productivity of the spinach industry.