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Anti-neoplastic compositions comprising extracts of black cohosh

a technology of black cohosh and composition, which is applied in the direction of drug compositions, biocide, tumor/cancer cells, etc., can solve the problems of insufficient study length, limited scope of foregoing studies, and little knowledge of the mechanism by which these compounds are metabolized, etc., to prevent neoplasia, treat or prevent neoplasia, and prevent neoplasia

Inactive Publication Date: 2012-08-30
EINBOND LINDA SAXE +6
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0014]In yet another aspect, the present invention provides a method for treating or preventing neoplasia in a subject, by administering to the subject an amount of an ethyl acetate extract of black cohosh effective to treat or prevent the neoplasia.
[0015]In still another aspect, the present invention provides a method for treating or preventing neoplasia in a subject, by administering to the subject an amount of an ethyl acetate extract of black cohosh effective to treat or prevent the neoplasia, in combination with an amount of at least one additional chemopreventive or chemotherapeutic agent effective to treat or prevent the neoplasia. In one embodiment of the invention, a synergistic anti-neoplastic effect results.

Problems solved by technology

Although most studies report that black cohosh is free of significant side-effects, these studies have not been carried out for a length of time sufficient to ensure the safety of black cohosh with respect to uterine function and / or the induction or stimulation of breast cancer growth.
However, little is known about the mechanisms by which these compounds are metabolized in vivo.
All of the foregoing studies, however, have been limited in scope, and have not addressed issues of specificity and mechanism of action.

Method used

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  • Anti-neoplastic compositions comprising extracts of black cohosh
  • Anti-neoplastic compositions comprising extracts of black cohosh
  • Anti-neoplastic compositions comprising extracts of black cohosh

Examples

Experimental program
Comparison scheme
Effect test

example 1

Chemicals and Reagents

[0097]Polyamide resin SC618CC silica gel (40 μM) was obtained from J. T. Baker (Phillipsburg, N.J.), and the RP18F254 plate (1-mm layer thickness) was obtained from EM Science (Darmstadt, Germany). Actein, 27-deoxyactein (23-epi-26-deoxyactein) (Zheng et al., CimiPure (Cimicifuga racemosa): a standardized black cohosh extract with novel triterpene glycoside for menopausal women. In Phytochem. Phytopharm., Shahidi and Ho, eds. (Champaign, Ill.: AOCS Press, 2000) pp. 360-70), cimifugoside, and cimiracemoside A were obtained from ChromaDex (Laguna Hills, Calif.), and 27-deoxyactein was also obtained from Herbstandard (Chesterfield, Mo.). Tamoxifen, 5-fluorouracil (5-FU), doxorubicin, cisplatin, and paclitaxel were purchased from Sigma (St. Louis, Mo.). Herceptin was obtained from Genentech (CA). Cimigenol and cimigenol glycoside were obtained from Dr. WC Ye (Department of Phytochemistry, China Pharmaceutical University, Nanjing 210009, China).

[0098]Black cohosh ex...

example 2

Plant Material

[0099]Black cohosh roots and rhizomes (GFP) were obtained from PureWorld Botanicals (South Hackensack, N.J.; lot number 9-2677).

example 3

Separation of the Ethyl Acetate Extract

[0100]As shown in FIG. 1, black cohosh roots and rhizomes were extracted with 80% methanol (MeOH) / H2O, and partitioned with n-hexane. Two layers were obtained: a water layer and an n-hexane layer. N-hexane was used to extract the non-polar phytochemicals, respectively, with yields of 0.05% hexane, 0.73% ethyl acetate, and 1.69% water. The water layer was partitioned with ethyl acetate, and two fractions were obtained: a water layer and an ethyl acetate layer. Ethyl acetate was used to extract the mid-polar and polar phytochemicals. The ethyl acetate layer was dried and evaporated to yield an ethyl acetate extract. The triterpene glycosides and cinnamic acid esters were separated from the ethyl acetate extract by polyamide chromatography (Kruse et al., Fukic and piscidic acid esters from the rhizome of Cimicifuga racemosa and the in vitro estrogenic activity of fukinolic acid. Planta. Med., 65:763-64, 1999).

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Abstract

A method for treating, preventing or ameliorating breast cancer is provided by administering a synergistic amount of digitoxin and either actein or an extract of black cohosh comprising triterpene glycosides, and optionally another chemopreventive agent which may be paclitaxel. Methods for treating or preventing a neoplasia using a synergistic combination, and compositions of a synergistic combination of a cardiac glycoside and either actein or an extract of black cohosh comprising triterpene glycosides, and optionally another chemopreventive agent which may be a taxane are also provided. The compositions may also be used in a method for modulating Na+K+ATPase activity. In addition, a method for inhibiting the progression or development of breast cancer in vivo by administering either actein or an extract of black cohosh comprising triterpene glycosides and optionally at least one other chemoprotective agent is provided.

Description

RELATED APPLICATIONS[0001]This application is a continuation-in-part of U.S. patent application deposited under Express Mail label No. EQ 523728379 US and having Docket No. IR 1390 CO35795 / 0225095, on Aug. 4, 2008 (informally designated U.S. Ser. No. 12 / 221,478), which is a divisional of U.S. patent application Ser. No. 10 / 746,960, filed on Dec. 23, 2003, which claims the benefit of U.S. Provisional Application Ser. No. 60 / 437,159, filed on Dec. 27, 2002, and entitled “ANTICANCER COMPOSITIONS OF EXTRACTS OF BLACK COHOSH”. The contents of these prior applications are incorporated herein by reference in their entireties.STATEMENT OF GOVERNMENT INTEREST[0002]This invention was made with government support under NIH Grant Nos. 3P50 AT 00090-02S2 and NIH NCCAM 5K01AT001692-03. As such, the United States Government has certain rights in this invention.BACKGROUND OF THE INVENTION[0003]Black cohosh, Actaea racemosa L. (Cimicifuga racemosa), a perennial in the buttercup family (Ranunculaceae...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K36/71C12N5/09C12N5/071A61P35/00A61K31/7048
CPCA61K31/7048A61K36/71A61K45/06A61K2300/00A61P35/00A61P35/02
Inventor EINBOND, LINDA SAXESOFFRITTI, MORANDOWEINSTEIN, I. BERNARDWEINSTEIN, JOANWEINSTEIN, TAMARAWEINSTEIN, CLAUDIAWEINSTEIN, MATTHEW
Owner EINBOND LINDA SAXE
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