Centrifugal micro-fluidic device and method for detecting analytes from liquid specimen

Abstract

A centrifugal micro-fluidic device detecting analytes in a liquid specimen and a method of detection of analytes from a liquid specimen using the micro-fluidic device are provided. Reaction efficiency is increased using a repetitive flow of the liquid specimen induced by an alternating combination of capillary force and centrifugal force, thereby enhancing detection sensitivity.

Description

BACKGROUND1. FieldApparatuses and methods consistent with exemplary embodiments relate generally to a centrifugal micro-fluidic device for detecting analytes in a liquid specimen and a detection method using the micro-fluidic device and, more particularly, to a centrifugal micro-fluidic device for detection of analytes from a liquid specimen with improved sensitivity. wherein a repeated flow of a liquid specimen induced by both capillary force and centrifugal force enhances reaction efficiency, as well as a method for detection of analytes in a liquid specimen using the micro-fluidic device.2. Description of the Related ArtIn order to cause a fluid to flow or move in a micro-fluidic structure of a micro-fluidic device, a driving pressure is generally required. The driving pressure may be a capillary pressure or pressure generated using an additional pump. In recent years, clinical diagnostic analyzers have been proposed that are designed to enable detection of a target material pres...

AI Technical Summary

Benefits of technology

The device achieves improved sensitivity and reaction efficiency through repeated fluid flow, reducing competitive inhibition and re-lysis issues, allowing for more effective detection and analysis of analytes.

Problems solved by technology

However, since the analyte is primarily combined with the labeling reagent, a large amount of labeling reagent is required, although this requirement is seldom satisfied in view of practical aspects.

Furthermore, combination of the analyte and each reagent is terminated within only a single fluid sample stream in one direction, thus resulting in insufficient combination and causing decrease in sensitivity and difficulties in quantitative analysis.

The kit does not have an active device for controlling a re-lysis rate of the detectable signal generator and, therefore, the detectable signal generator is excessively re-lysed by a constant volume of a fluid sample flowing thereto, thus causing waste of the detectable signal generator at an early stage.

On the other hand, re-lysis of the detectable signal generator is drastically reduced in a later stage, thus entailing difficulties in sensitive detection of the analyte.

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