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DWF12 and Mutants Thereof

a technology of dwf12 and mutants, applied in the field of plants, can solve the problems of difficult to obtain additional components of br signaling, and achieve the effect of short robust stature and reduced or increased shaggy kinase activity

Inactive Publication Date: 2009-01-22
THE ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIV OF ARIZONA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention relates to molecules involved in the brassinosteroid (BR) signaling pathways and mutants thereof. The inventors have performed extensive screening of EMS-mutant populations and isolated novel BR-insensitive mutants. These mutants display a dwarf phenotype, similar to other BR mutants, but with more severe phenotypes. The inventors have identified DWF12, a conserved protein predicted to reside in the cytoplasm, and have shown that it encodes a serine / threonine protein kinase. The inventors have also identified mutants that have one or more mutations in conserved sequences. These mutants can be used to produce transgenic plants with altered phenotypes. The technical effect of the invention is the identification of new components of the BR signaling pathways and mutants thereof, which can help to better understand and modulate the BR signaling cascade in plants.

Problems solved by technology

Compared to the flurry of BR biosynthetic mutants, a dearth of mutants defective in the BR signaling pathway has made it difficult to obtain additional components of BR signaling.

Method used

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  • DWF12 and Mutants Thereof

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example 1

Phenotypes of dwf12 Mutants

[0169]dwf12-1 and dwf12-2 plants were generated by EMS (ethylmethanesulfonate) mutagenesis using techniques well known in the art. The mutants were identified due to their characteristic dwarf phenotype. dwf12-1 and dwf12-2 plants were compared to a BR-biosynthetic mutant, dwf4-1, and BR-insensitive mutants bri1 and twisted (twd). The phenotypes of the two dwf12 alleles were similar to dwf4 and bri1 plants in that they all displayed dark green and round leaves, short and sturdier stature relative to wild type (Ws-2). A unique phenotype found only in dwf12 mutants was severe downward curling of leaves. Plants heterozygous for dwf12 mutations showed a semi-dwarf phenotype with a height intermediate between wild-type and homozygous mutant plants, suggesting that the dwf12 mutations are dominant-negative, gain of function, or haploinsufficient. Further morphometric analysis of the two dwf12 alleles, dwf12-1 and dwf12-2, indicated that the dwf12-2 mutations res...

example 2

Ectopic Overexpression of DWF12

[0170]Previously, we found that ectopic overexpression of the DWF4 gene encoding a rate-limiting step enzyme in the BR biosynthetic pathways resulted in bigger plants due to elongated cell size. Thus, the same approach was used to examine the effects contributed by DWF12 overexpression. A vector comprising the 35S promoter operably linked to wild-type DWF12 cDNA was compared to a vector control harboring only a vector. 11 independent transformants (AOD12) carrying the DWF12 overexpression construct consistently displayed a bigger plant phenotype, such as long petiole, elongated leaf blades, bigger flowers and weaker inflorescences. The bigger plant phenotypes of AOD12 suggests that the DWF12 gene is transcriptionally controlled, and this regulation is important in determining the cell sizes and whole plant morphology in Arabidopsis.

[0171]Thus, DWF12 sequences, polypeptides, mutants thereof and uses of these molecules are described. From the foregoing,...

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Abstract

DWARF12 (DWF12) sequences, mutants and methods of using the same are disclosed. The dwf12 polynucleotides can be used in the production of transgenic plants which display at least one dwf12 mutant phenotype, so that the resulting plants have altered biochemistry, structure or morphology.

Description

TECHNICAL FIELD[0001]The present invention relates generally to plants that display altered biochemical properties, structure or morphology and to the genes imparting such phenotypes. In particular, the present invention pertains to DWARF12 (DWF12), dwf12 mutants and methods of using the same.BACKGROUND OF THE INVENTION[0002]The brassinosteroids (BRs) are a group of plant steroid hormones that help regulate many different aspects of plant growth and development. BRs are known to stimulate cell elongation and division, and are also involved in vascular system differentiation, reproduction, and stress responses (Altmann, 1998; Clouse and Sasse, 1998). Recently, it has been shown that mutants defective either in BR biosynthesis or signaling, display altered developmental phenotypes including dwarfism, reduced fertility, and abnormal vasculature (Clouse and Feldmann, 1999).[0003]BR dwarf mutants can be divided into two classes. The first class of mutants is perturbed in BR signaling. Fo...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/82C07K14/415C07H21/04C12N15/63C12N5/02C12N5/00C12P21/06A01H5/00C12N9/12
CPCC12N9/1205C12N15/829C12N15/8261Y02A40/146
Inventor CHOE, SUNGHWAFELDMANN, KENNETH A.TAX, FRANS
Owner THE ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIV OF ARIZONA
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