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Anti-CCR4 antibodies and methods of use therefor

a technology of anti-ccr4 and antibodies, which is applied in the field of anti-ccr4 antibodies, can solve the problems of determining the relevance of disease initiation and progression, and elucidating the normal immune function of a specific receptor on a given cell typ

Inactive Publication Date: 2007-02-08
WU LIJUN +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This makes elucidating the normal immune function for a specific receptor on a given cell type and determining the relevance to initiation and progression of disease difficult, especially since specific antibodies are not available for many chemokine receptors.

Method used

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  • Anti-CCR4 antibodies and methods of use therefor
  • Anti-CCR4 antibodies and methods of use therefor
  • Anti-CCR4 antibodies and methods of use therefor

Examples

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Materials and Methods

Construction of CCR4 Stable Transfectants

[0112] CCR4 cDNA was obtained by PCR using a 5′-oligonucleotide primer (5′-CCAACCAAGCTTATGAACCCCACGGATATAGCAG-3′; SEQ ID NO: 1) and 3′-oligonucleotide primer (5′-CCAACCTCTAGATTAGAGCATCATGGAGATCATGATCC-3′; SEQ ID NO: 2) which contained flanking HindIII and XbaI sites, respectively. The PCR fragment was subcloned into the Hindifi and XbaI sites of pMRB 101, in which the inserted gene was driven by a CMV promoter. The DNA was stablely transfected into a murine pre-B lymphoma cell line (L1.2 or L1 / 2) as described (Ponath et al., J. Exp. Med. 183:2437 (1996); Wu et al., J. Biol. Chem. 271:31202 (1996); Wu et al., Nature 384:179 (1996)). The cells that expressed high levels of CCR4 were selected by serial dilution / subcloning for their ability to chemotax to TARC and MDC. For monoclonal antibody production, the cells were treated with 5 mM butyric acid for 16-18 hours and used for immunizing mice.

Cells and Cell Lines

[0113...

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Abstract

The present invention relates to an antibody or functional fragment thereof which binds to a mammalian (e.g., human) CC-chemokine receptor 4 (CCR4) or a portion of the receptor and blocks binding of a ligand to the receptor. The invention further relates to a method of inhibiting the interaction of a cell bearing mammalian CCR4 with a ligand thereof, and to use of the antibodies and fragments in research, therapeutic, prophylactic and diagnostic methods.

Description

RELATED APPLICATIONS [0001] This application is a continuation of U.S. application Ser. No. 10 / 055,789, Jan. 18, 2002, which is a continuation of U.S. application Ser. No. 09 / 871,239, filed May 30, 2001, which is a continuation-in-part application of U.S. application Ser. No. 09 / 483,951, filed Jan. 14, 2000, which is a continuation-in-part application of U.S. application Ser. No. 09 / 231,759, filed Jan. 15, 1999, the entire teachings of which are incorporated herein by reference in their entirety.BACKGROUND OF THE INVENTION [0002] Over the last decade chemokines have emerged as key mediators of inflammation as a result of their numerous proinflammatory activities which affect virtually every leukocyte type. More recently, chemokines have been recognized as a critical component of basal leukocyte trafficking essential for normal immune surveillance and response, as well as for several other functions in hematopoiesis, angiogenesis, control of viral infection, and T cell differentiatio...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/567C12N5/06C07K16/24G01N33/532A61K39/395A61P25/00A61P43/00C07K16/28C12N5/10C12N5/20C12P21/08G01N33/566G01N33/577
CPCC07K16/2866A61K2039/505A61P25/00A61P43/00
Inventor WU, LIJUNRUFFING-GRAHAM, NANCYANDREW, DAVID P.
Owner WU LIJUN
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