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Differential diagnosis of cancer and other conditions based on expression of p63

a technology of p63 and differentiation diagnosis, applied in the field of gene products, can solve the problems that p63 does not yet have a defined role in neoplasia and other physiological and pathological situations

Inactive Publication Date: 2006-02-16
MT SINAI SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides methods for detecting p63 nucleic acid and protein expression in cells or tissues for diagnostic and prognostic purposes. These methods include nucleic acid hybridization, PCR, and RT-PCR, as well as protein assays using antibodies that bind to p63 polypeptides. The invention also provides a kit comprising the reagents required for detection of p63 nucleic acids or polypeptides. The invention also provides methods for distinguishing different types of lung cancer and distinguishing thyroid papillary carcinoma from other thyroid disorders by detecting p63 expression. The invention also provides a method for treating lung cancer by diagnosing it as a squamous cell carcinoma and surgical resection of the p63-expressing tumor.

Problems solved by technology

However, p63 does not yet have a defined role in neoplasia and other physiological and pathological situations (Hall et al., supra).

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Immunohistochemical Study of Expression of p53-Homolog p63 in Pulmonary Neoplasms

[0067] p63 is a nuclear protein recently discovered in a screen for genes with homology to the tumor suppressor p53 gene. This Example describes p63 expression in benign lung tissue and neoplasms of pulmonary origin.

Materials and Methods

[0068] Tissues. Archival, routinely processed, formalin-fixed, paraffin-embedded surgical pathology specimens from bronchoscopic biopsy and pneumonectomy and / or lobectomy specimens were examined.

[0069] Antibody. The antibody used in this study was anti-p63 monoclonal antibody 4A4 (Santa Cruz Biotechnology, Inc., Santa Cruz Calif.). This antibody is reactive against a region common to both the full-length and truncated forms of p63.

[0070] Immunohistochemical staining. Five-micron-thick sections were heated and deparaffinized and were treated with 0.3% hydrogen peroxide to block endogenous peroxidase activity and pretreated with citric acid (pH 6.0) for antigen retrie...

example 2

Expression of p63 Protein in Subtypes of Transitional Cell and Renal Cell Carcinomas

[0089] The p53 family of tumor suppressor genes includes p63, which is highly expressed in the basal layers of epithelial tissues. P63 may either promote apoptosis or antagonize p53, depending upon the expressed protein isoform. Expression of p63 has been found in transitional epithelium. Expression of p63 in transitional cell carcinomas was compared with expression in renal cell carcinomas. Non-cancerous tissues associated with the urinary tract were also examined.

Methods

[0090] Formalin-fixed, paraffin-embedded archival tissue from 81 patients was immunostained with p63 monoclonal antibody 4A4 (Santa Cruz) reactive against all subtypes of p63 protein as described above for Example 1. Immunostaining patterns and routine H&E staining patterns were interpreted by four observers. Nuclear staining was considered positive.

Results

[0091] Normal tissue. Benign adrenal cortex (1), prostate (2), and other...

example 3

Expression of p63 in Papillary Thyroid Carcinoma and in Hashimoto's Thyroiditis

[0095] Nuclear proteins were discovered as a consequence of a screen for p53-homologous genes. Thus far, p63 expression has been demonstrated in basal cells of squamous epithelium and in urothelium. Immunohistochemical detection of p63 expression was evaluated in normal and abnormal thyroid tissue.

Materials and Methods

[0096] Five micron sections from routinely fixed and processed archival thyroid resection specimens were pretreated with citric acid pH 6.0 for antigen retrieval at 100° C. for 5 minutes, then incubated overnight with anti-p63 monoclonal antibody 4A4 (Santa Cruz). Slides were stained using a streptavidinbiotin kit (BioGenex), followed by reaction with diaminobenzidine, and then counterstaining with hematoxylin.

Results

[0097] Conditions negative for p63. No p63 expression was seen in normal thyroid tissue, twelve follicular adenomata, eleven Hurthle cell adenomata, six medullary carcinoma...

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Abstract

This invention relates to methods of distinguishing among various types of differentiated and undifferentiated epithelial carcinomas, and non-epithelial carcinomas, by detecting the presence of p63 nucleic acid or protein expression. The invention also provides methods for detecting p63 nucleic acids and proteins, as well as methods for diagnosing and treating certain tumors based on whether the tumors express p63.

Description

[0001] This Application is a Division of application Ser. No. 10 / 043,502 filed on Jan. 9, 2002. Ser. No. 10 / 043,502 is a Non-Provisional of Provisional (35 USC 119(e)) application 60 / 261,603 filed on Jan. 12, 2001.[0002] The research leading to the present invention was supported, in part, by the National Cancer Institute Grant No. R21-CA81362. Accordingly, the U.S. Government may have certain rights in the invention.FIELD OF THE INVENTION [0003] The present invention relates to a gene product, referred to herein as p63, and to nucleic acids, including the gene, mRNAs, and cDNAs, encoding it, in particular, to methods of using the gene and gene product to diagnose and / or treat certain diseases and disorders such as cancer. The invention also relates to kits which may be used in such diagnostic and treatment methods. BACKGROUND OF THE INVENTION [0004] The nuclear protein p63, located on chromosome 3q, has been shown to possess homology with p53, a tumor-suppressor gene encoding a mul...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01N33/574
CPCG01N33/57423G01N33/57484G01N2800/7095G01N33/564G01N2800/046G01N33/5748
Inventor BURSTEIN, DAVID E.
Owner MT SINAI SCHOOL OF MEDICINE
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