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Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items

a technology of renilla reniformis and fluorescent proteins, which is applied in the field of renilla reniformis fluorescent proteins, can solve the problems that the gfp, however, is not ideal for use in analytical and diagnostic processes, and achieves the effect of higher outpu

Inactive Publication Date: 2005-12-08
GAUS
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  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

The patent text describes the isolation and use of nucleic acids and proteins from a sea creature called Renilla reniformis, which can be used to create a bioluminescence generating system. This system can be used in various applications, such as in diagnostics and for creating novelty items. The patent also describes the use of mutated forms of the proteins to alter their emission properties. Overall, the patent provides a way to create a unique and interesting system for generating light in cells and animals.

Problems solved by technology

The A. victoria GFP, however, is not ideal for use in analytical and diagnostic processes.

Method used

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  • Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
  • Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
  • Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items

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[0507] Specimens of the sea pansy Renilla reniformis were collected from inshore waters off the coast of Georgia. To prepare the sea pansies for isolation of mRNA, about 25 or so at time were placed on a large bed of dry ice. They were flipped with a spatula to flip them over to prevent them from freezing. Oddly, the entire animal illuminated when it came in contact with the dry ice. The brightest and greenest were culled, placed in a bag and back into sea water at about 65-70° C. for two hours. This process of dry ice, culling and sea water treatment was repeated three times over a 6 hour period. In addition, the process was performed at night. After they were exhausted with the last chilling, the culled animals were frozen solid. A cDNA library was prepared from the frozen animals.

[0508] The animals that were selected this way were frozen in liquid nitrogen, and shipped to Stratagene, Inc. (La Jolla, Calif.), a commercial vendor whose business includes the construction of custom ...

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Abstract

Isolated and purified nucleic acids encoding green fluorescent proteins from Renilla reniformis and the green fluorescent protein encoded thereby are also provided. Mutants of the nucleic acid molecules and the modified encoded proteins are also provided. Compositions and combinations comprising the green fluorescent proteins and / or the luciferase are further provided.

Description

RELATED APPLICATIONS [0001] This application is a divisional of allowed U.S. patent application Ser. No. 09 / 808,898 filed Mar. 15, 2001. Benefit of priority is also claimed under 35 U.S.C. §119(e) to U.S. Provisional Application Ser. No. 60 / 189,691, filed Mar. 15, 2000, to Bryan et al., entitled “RENILLA RENIFORMIS FLUORESCENT PROTEINS, NUCLEIC ACIDS ENCODING THE FLUORESCENT PROTEINS AND THE USE THEREOF IN DIAGNOSTICS, HIGH THROUGHPUT SCREENING AND NOVELTY ITEMS”. [0002] This application is related to U.S. patent application No. 09 / 277,716, filed Mar. 26, 1999, to Bruce Bryan and Christopher Szent-Gyorgyi, entitled “LUCIFERASES, FLUORESCENT PROTEINS, NUCLEIC ACIDS ENCODING THE LUCIFERASES AND FLUORESCENT PROTEINS AND THE USE THEREOF IN DIAGNOSTICS, HIGH THROUGHPUT SCREENING AND NOVELTY ITEMS”, now U.S. Pat. No. 6,232,107. This application is related to International PCT Application No. WO 99 / 49019 to Bruce Bryan and Prolume, LTD., entitled “LUCIFERASES, FLUORESCENT PROTEINS, NUCLEIC...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C07K14/435C12N9/02
CPCC07K14/43595C07K2319/00C12N9/0069C12Y113/12005
Inventor BRYAN, BRUCESZENT-GYORGYI, CHRISTOPHERSZCZEPANIAK, WILLIAM
Owner GAUS
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