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Peptides with wound healing activity

a technology of peptides and wounds, applied in the field of wound healing, can solve the problems of cosmetically undesirable consequences, scarring and impairment of function, and the healing of wounds still presents a considerable challenge to the clinician, and achieves the effects of stimulating the immune system, stimulating pituitary/hypothalamus activity, and promoting wound healing

Inactive Publication Date: 2005-11-10
ZAVERI CHANDA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In chronic wound healing, there is typically an absence of epithelial migration, excessive granulation tissue, and fibrosis, with scarring and impaired function possibly being present.
Although many advances have been made in the understanding of wound healing, the healing of wounds still presents a considerable challenge to the clinician.
Additionally, when such wounds do heal, they frequently heal with cosmetically undesirable consequences such as scars or discoloration.

Method used

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  • Peptides with wound healing activity
  • Peptides with wound healing activity
  • Peptides with wound healing activity

Examples

Experimental program
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Effect test

example 1

[0099] Effects of TB4 and TA on Endothelial Cell Migration

[0100] The effects of TB4 and TA1 on endothelial cell migration were determined. To investigate the possibility that these peptides effect the migration of normal human vascular endothelial cells (NHVEC) in vitro, a Boyden chamber migration assay was performed.

[0101] The results of these are shown in Table 1 and in FIG. 1. In the experiments of Table 1 and FIG. 1, normal human microvesicular endothelial cells are used at 5×104 cells per well. The wells are coated with 100 μg / ml bovine serum albumin Type IV.

[0102] These results demonstrate that both TB4 and TA1, act as chemoattractants for endothelial cells, stimulating the migration of NHVECs in Boyden chambers. At concentrations of 100 ng / ml, both TB4 and TA1, significantly enhanced cell migration over migration in the presence of media alone. Notably, TB4 and TA1, also revealed heightened responses at this concentration when compared to three positive controls: vascular ...

example 2

[0103] Effects of TB4 and TA1, on Endothelial Cell Proliferation

[0104] The effects of TB4, and TA1, on endothelial cell proliferation were determined using a MTT (tetrazolium) assay. The results are shown in FIG. 2 for 4 hours and 24 hours and for three concentrations of these peptides, 10 ng / ml, 100 ng / ml, and 1000 ng / ml. These results show that these peptides stimulate endothelial cell proliferation. At a concentration of 10 ng / ml, TB4 and TA1 maximally stimulated cell proliferation approximately 2-fold and 3-fold over that of the control, respectively.

example 3

[0105] Effects of TB4 and TA1 on Endothelial Cell Proliferation

[0106] To examine the effects of TB4 and TA1 (10 ng / ml) on NHVEC migration, a scratch wound closure assay was used. When a confluent monolayer was scratched, migration of cells into the wounded area was significantly increased in the presence of TB4 and TA1 compared to migration with media alone.

[0107] Thus, these peptides significantly enhance endothelial cell migration, proliferation, and angiogenesis, and may play an important role in the wound healing process.

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Abstract

A number of peptides of from 28 to 44 amino acids, including therein a sequence of L-K-E-K-K (SEQ ID NO: 1) have a physiological activity that can be wound healing activity, immunostimulant activity, or growth factor activity. The peptides can be linear or circular, and if linear, the amino terminus can be optionally acetylated. The invention also includes nucleic acids encoding these peptides, vectors incorporating these nucleic acid sequences, and host cells transfected with these vectors, as well as methods for producing these peptides by culturing these host cells and purifying these peptides. The invention further includes pharmaceutical compositions and methods for using these peptides.

Description

CROSS-REFERENCES [0001] This application is a divisional application of application Ser. No. 09 / 879,666, by Chanda Zaveri, entitled “Peptides with Wound Healing Activity” (as amended), filed Jun. 12, 2001, which in turn claimed priority from Provisional Application Ser. No. 60 / 211,859 by Chanda Zaveri, filed Jun. 14, 2000, entitled “Peptides with Physiological Activity,” both of which are incorporated herein in their entirety by this reference.BACKGROUND OF THE INVENTION [0002] This invention is directed to peptides with various physiological activities, particularly wound healing. [0003] Wound healing is a complex biological process that differs according to the wound type: acute or chronic. The principal elements of wound repair are the immediate events of hemostasis and stimulus for inflammation, then inflammation and cell proliferation and migration, then followed by molecular synthesis, collagen polymerization and cross-linking, remodeling, and wound contraction. Inflammation i...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/00C07K14/515
CPCC07K14/515A61K38/00
Inventor ZAVERI, CHANDA
Owner ZAVERI CHANDA
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