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Process for producing high purity isoflavones

Inactive Publication Date: 2004-09-30
ARCHER DANIELS MIDLAND CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0007] The present invention provides a simple and effective method to produce high purity isoflavones from soy solubles. Specifically, the present invention provides a process for producing high purity isoflavone fractions from a plant starting material, said process comprising the steps of subjecting the plant material to a primary chromatographic step to obtain an isoflavone enriched fraction and subjecting the isoflavone enriched fraction to a second chromatographic step. The present invention also provides a process for producing high purity isoflavone fractions from a plant material comprising the step of passing the plant material through an ultrafiltration membrane which has a moleuclar weight cut-off range that produces a plant material permeate and subjecting the plant material permeate to a primary chromatographic step to obtain an isoflavone enriched fraction and subjecting the isoflavone enriched fraction to a second chromatographic step.

Problems solved by technology

All of these reported processes are too cumbersome for the production of isoflavones on an industrial scale.
Also, these processes are unable to make a high purity product.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 2

Primary Chromatography

[0020] This experiment was the similar to that described in Example 1 with the following exceptions:

[0021] 1. Feed material was pH adjusted to 8.6.

[0022] 2. Feed volume was 20 bed volumes.

[0023] 3. Ethanol solution was of 70% concentration.

[0024] The results from this operation appear below:

2 Isoflavones Dry Solids Purity Yield SAMPLE (mg / Kg) (g / Kg) (%) (%) FEED 544.9 47.1 1.2 PRODUCT 857.4 1.4 63.0 78.7

example 3

Primary Chromatography

[0025] This experiment was the similar to that described in Example 2 with the following exceptions:

[0026] 1. Feed material was pH adjusted to 9.0.

[0027] 2. Resin used was ROHM & HAAS XAD4.

[0028] The effluent streams from this operation appear below:

3 Isoflavones Dry Solids Purity Yield SAMPLE (mg / Kg) (g / Kg) (%) (%) FEED 1773.7 203.7 0.9 PRODUCT 5476.7 10.8 50.9 77.2

example 4

Secondary Chromatography

[0029] Experiments were carried out in a jacketed glass column containing 100 mls of non-ionic adsorbent resin (in this case TULSION ADS 600). The temperature of the column is maintained at 60-65.degree. C. by circulating hot water through the jacket of the column. The resins were conditioned by running 3 bed volumes (300 mls) of a solution of 2.5% NaOH through the resin. The resins were then rinsed with 3 bed volumes of deionized water. The flow rate for all steps in this test was 12 mls / min. Ethanol from the product made by the primary chromatography was used as the starting feed in these tests. This feed was diluted with water and the pH was adjusted to 9.3. One liter of this feed material was then passed through the resin bed. Then 3 bed volumes of deionized water was passed through the column. After the rinse, 5 bed volumes (500 mls) of a solution of 35% ethanol was passed through the resin. The effluent was sampled and collected as PROD. FRAC1. Then 5 b...

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Abstract

A process for the production of a high purity isoflavone composition is disclosed. The process involves subjecting plant material to a primary chromatographic step to obtain an isoflavone enriched fraction and subjecting the isoflavone enriched fraction to a second chromatographic step. Also disclosed is a process that invloves passing the plant material through an ultrafiltration membrane which has a molecular weight cut-off range that produces a plant material permeate prior to subjecting the plant material permeate to primary and secondary chromatographic steps.

Description

[0001] This application claims benefit of U.S. Provisional Application No. 60 / 371,129, filed Apr. 10, 2002, which is incorporated herein by reference in its entirety.[0002] 1. Field of the Invention[0003] The present invention relates to a process for producing high purity isoflavones. More particularly, the present invention relates to a process for producing high purity isoflavones by a process combining primary and secondary chromatographic steps.[0004] 2. Related Art[0005] Isoflavones are a class of plant flavonoid compounds which have estrogenic activity. Research has revealed many possible health benefits that may be achieved from the consumption of isoflavones. High cholesterol is believed to contribute to the development of coronary heart disease and isoflavones have been demonstrated to lower cholesterol in animal studies. Isoflavones may also play a role in preventing certain types of cancer. Bone loss in women during and after menopause may also be prevented or ameliorate...

Claims

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Application Information

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IPC IPC(8): C07D311/36
CPCC07D311/36
Inventor HILALY, AHMAD K.SANDAGE, BOBSOPER, JOHN
Owner ARCHER DANIELS MIDLAND CO
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