Flow cytometry and micro-carrier gene chip

Abstract

The invention discloses a high-flux typed analyzing technique, material and agent box of nucleic acid based on stream-oriented cell tool-microcarrier gene chip, which is characterized by the following: augmentating promoter starting target to connect biotin; connecting magnet microparticle and fluorescent material on the nucleic acid probe; crossing with single-chain of target nucleic acid; separating and purifying target nucleic acid single-chain-probe; adopting high-polymolecule microparticle of clad biotin-proof protein as microcarrier; connecting target nucleic acid single-chain-probe; adopting double probe-sandwich method to test nucleic acid single-chain.

Description

Technical field [0001] The invention relates to the technology, material and kit for "high-throughput" analysis of nucleic acid using flow cytometer-microcarrier gene chip. Background technique [0002] With the rapid development of molecular biology, nucleic acid analysis techniques based on PCR and nucleic acid hybridization are playing an increasingly important role in disease diagnosis. These techniques are extremely sensitive, with reactions initiated in the presence of 10-20 pairs of nucleic acid strands (RNA or DNA). Taking infectious diseases as an example, nucleic acid analysis technology is useful for early diagnosis, pathogen typing, and Monitoring the outbreak plays a pivotal role. These effects cannot be replaced by conventional antigen and antibody detection methods. [0003] The classic PCR technology uses the nucleic acid strand (RNA or DNA) of the sample to be tested as a template, and under the action of transcriptase, reverse transcriptase, or nucleic a...

AI Technical Summary

Problems solved by technology

Firstly, single-strand nucleic acid to be tested lacks effective isolation and purification, poor specificity, and high background staining; secondly, PCR and flow cytometry analysis in a test tube are generally monovalent, that is, for one target nucleic acid, so the efficiency is low

These disadvantages limit the application of this technology

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