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Freely diffusible eutelegenesis for aquatic animal

An aquatic animal and free technology, applied in the field of free diffusion artificial insemination, can solve the problem of sperm reduction and other problems

Inactive Publication Date: 2007-05-09
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a free method that is essentially different from the other three artificial insemination methods for the existing artificial insemination methods for aquatic animals that ignore the phenomenon that the vitality of the sperm is reduced to varying degrees after cryopreservation. diffusion artificial insemination

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The cryovial containing 1 mL of large yellow croaker semen was taken out of the liquid nitrogen and placed in seawater at room temperature for rapid thawing. Pour the thawed semen into the bottom of the moist container and quickly feather it. Slowly pour 150mL room temperature seawater into the container and let it stand for 2min. The supernatant seawater containing a large amount of healthy sperm is slowly poured into the container containing fresh eggs, and gently stirred to complete the insemination. 10 minutes after insemination, the fertilized eggs were rinsed with a large amount of seawater to remove excess semen.

Embodiment 2

[0019] Similar to Example 1, the difference is that the freezing tube containing 1 mL of large yellow croaker semen was taken out of the refrigerator, and placed in seawater at room temperature for rapid thawing. Pour the thawed semen into the bottom of the moist container and quickly feather it. Slowly pour 100mL room temperature fresh water into the container and let it stand for 3min.

Embodiment 3

[0021] Similar to Example 1, the difference is that the cryovial containing 1 mL of large yellow croaker semen was taken out of the liquid nitrogen and placed in seawater at room temperature for rapid thawing. Pour the thawed semen into the bottom of the moist container and quickly feather it. Slowly pour 200mL room temperature seawater into the container and let it stand for 3min.

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Abstract

A free diffusion method for artificial insemination of aquatic animal includes such steps as thawing the frozen seminal liquid of aquatic animal, using soft material to coat it onto the bottom of wet container, adding fresh water or sea water to activate the spermatozoa for allowing the health spermatozoa to swim in solution, taking the upper-layer solution, mixing it with fresh ova, inseminating, and rinsing with sea water to remove excessive seminal liquid.

Description

technical field [0001] The invention relates to an artificial insemination of aquatic animals, in particular to a free diffusion artificial insemination method suitable for cryopreservation or poor vitality of aquatic animal sperm. Background technique [0002] With the development of modern biotechnology, people are increasingly using sperm cryopreservation to make sperm and eggs isolated in time or place can be fertilized normally. This method is especially commonly used in the breeding operations of aquatic animals. However, in actual operation, the fertilization rate and hatching rate of eggs generally decrease. Many researchers start with the formula of sperm cryopreservation solution in order to improve the fertilization rate and hatching rate of fertilized eggs, but the improved formula of sperm cryopreservation solution can only slightly increase the fertilization rate and hatching rate of fertilized eggs. Another concern is the method of artificial insemination. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D19/02
Inventor 王德祥苏永全王军
Owner XIAMEN UNIV
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