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Pupa cordyceps fruiting body cultured using large galleria waxmoth larva and its culturing method

A cultivation method, the technology of the wax moth, which is applied in the direction of fungi, etc., can solve the problems that there is no use of the larvae of the wax moth to cultivate the fruiting bodies of Cordyceps

Inactive Publication Date: 2007-04-18
INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, all do not see the report that utilizes greater wax moth larvae to cultivate Cordyceps fruiting bodies so far

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] With potato 20% (mass fraction, the same below), glucose 2%, KH 2 PO 4 0.3%, MgSO 4 .7H 2 O0.15%, Vitamin B 1 20mg / L, 2% agar, and 0.5% of the larvae of Mellonella mellonella were prepared as a primary medium, with 2% glucose, 0.4% peptone, and KH 2 PO 4 0.4%, MgSO 4 .7H 2 O 0.4%, Vitamin B 1 20mg / L, 0.5% of the larvae of Mellonella mellonella, pH 6.5 to prepare the secondary medium.

[0025] Inoculate the isolated and purified Cordyceps militaris strains into the primary culture medium on a solid slant, incubate at 20°C in the dark for 7 days, until the white fluffy hyphae cover the slope, and then connect the primary strains from the plate to the sterilized In the liquid culture medium of the second-grade bacterial species, shake culture at 25°C and 120r / min for 7 days until the bacterial balls are filled with the liquid culture medium. The larvae of Mellonella mellonella were sterilized with ethanol with a mass fraction of 75%, and then 0.01 mL of bacteria ...

Embodiment 2

[0028] With potato 20%, glucose 2%, KH 2 PO 4 0.3%, MgSO 4 .7H 2 O0.15%, Vitamin B 1 20mg / L, 2% agar, and 5.0% of the larvae of Mellonella mellonella were used to prepare the primary medium, with 2% glucose, 0.4% peptone, and KH 2 PO 4 0.4%, MgSO 4 .7H 2 O0.4%, Vitamin B 1 20mg / L, 5.0% of the larvae of Mellonella mellonella, pH 6.5 to prepare the secondary medium.

[0029] Inoculate the isolated and purified strains of Cordyceps militaris to the primary culture medium on a solid slant, and culture them in the dark at 25°C for 10 days until the white fluffy hyphae cover the slope, and then connect the primary strains from the plate to the sterilized In the liquid culture medium of the secondary strains, shake culture at 25°C and 120r / min for 10 days, until the balls are filled with the liquid medium, and then inject 0.2mL of the strains into the larvae of the artificially cleaned and reared Great Melonella moth with a sterile syringe .

[0030] Place the infected la...

Embodiment 3

[0032] With potato 20%, glucose 2%, KH 2 PO 4 0.3%, MgSO 4 .7H 2 O0.15%, Vitamin B 1 20mg / L, 2% agar, 3.0% substance of Mellonella mellonella larvae to prepare primary medium, with 2% glucose, 0.4% peptone, KH 2 PO 4 0.4%, MgSO 4 .7H 2 O0.4%, Vitamin B 1 20mg / L, 3.0% of the larvae of Mellonella mellonella, pH 6.5 to prepare the secondary medium.

[0033] Inoculate the isolated and purified Cordyceps militaris strains into the primary culture medium on a solid slant, and incubate for 8 days in the dark at 23°C until the white fluffy hyphae cover the slope, and then connect the primary strains from the plate to the sterilized In the liquid culture medium of the second-grade bacterial species, shake culture at 25°C and 120r / min for 8 days until the bacterial balls are filled with the liquid culture medium. The surface of the larvae of Mellonella mellonella was disinfected with ethanol with a mass fraction of 75%, and then the larvae of Mellonella mellonella were soaked w...

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Abstract

This invention relates to a cordyceps militaris (L.) link encarpium which is raised by Galleria mellonella L larva and its cultural method. The characteristic of this invented Cordyceps encarpium is that Galleria mellonella L larva shows Buff, body surface has little orange yellow bacterial filament, encarpium is Club shape or cladodromous, orange yellow, one or more encarpium grow thickly from body surface of Galleria mellonella L larva pupa, and after Cordyceps militaris(L.)Link strain is cultivated by first class strain cultivation and second order strain cultivation, using second order liquid strain to infect Galleria mellonella L larva, and it is obtained by raising encarpium. This invented Cordyceps militaris(L.)Link encarpium is similar to wild Cordyceps militaris(L.)Link, nutritional ingredient of them is similar.

Description

technical field [0001] The invention relates to a fruiting body of Cordyceps militaris, in particular to a fruiting body of Cordyceps militaris cultivated by larvae of the mellonella mellonella, and also to a method for cultivating the fruiting body of the Cordyceps militaris. Background technique [0002] Cordyceps militaris (L.) Link, also known as Cordyceps militaris, belongs to the phylum Ascomycota, the class Ascomycetes, the order Hyoceales, the family Clavicipitaceae, and the genus Cordyceps. Cordyceps militaris mainly parasitizes the pupae of Lepidoptera insects in nature. Cordyceps militaris is widely distributed all over the world, and is distributed in all provinces of my country. [0003] Cordyceps sinensis (C. sinensis (Berk) Sacc.), which belongs to the same genus as Cordyceps militaris, is a traditional Chinese medicine resource, which has health care and medicinal value, especially anti-tumor effect. The nutritional components and medicinal value of Cordyce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 韩日畴刘小芬曹莉陈镜华
Owner INST OF ZOOLOGY GUANGDONG ACAD OF SCI
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