Method of cultivating bacterial spawn for umbellate pore fungus
A cultivation method, the technology of Polyporus chinensis, applied in the field of microbial cultivation, can solve the problems of extremely high cultivation conditions, difficult management, and high wood consumption, and achieve the effects of shortening cultivation time, reducing production costs, and increasing production
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[0018] 1. Preparation of strains:
[0019] (1) First prepare the solid culture medium (PDA) for separating Polyporus umbilicalis, separate the tissues of the wild Polyporus umbellata and the planted Polyporus umbellata respectively, and select excellent hyphae without miscellaneous bacteria respectively, and hybridize the above two strains to prepare Become the first-class Polyporus mother species, and then make the fungus branch of Amygdalus aeruginosa.
[0020] (2) Prepare the secondary bacterial culture medium, use 15% Maifuzi, 84% broad-leaved sawdust, and 1% white sugar to make the secondary bacterial culture medium, add water and stir evenly, and make the moisture content of the medium reach 55% -60%, put it into a bottle, plug it with a cotton plug, and sterilize it under normal pressure for more than 8 hours, take it out of the pot to cool down the bottle, and when the temperature of the culture medium reaches 28°C, insert the first-grade strain of Polyporus chinensis ...
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