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Method of cultivating bacterial spawn for umbellate pore fungus

A cultivation method, the technology of Polyporus chinensis, applied in the field of microbial cultivation, can solve the problems of extremely high cultivation conditions, difficult management, and high wood consumption, and achieve the effects of shortening cultivation time, reducing production costs, and increasing production

Inactive Publication Date: 2007-04-18
董庆忠
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  • Summary
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AI Technical Summary

Problems solved by technology

There are two ways of artificial planting, one is to use sclerotia to cultivate, but it takes 4-5 years to harvest, and the survival rate of cultivation is only about 60%, which consumes a lot of wood and is difficult to manage. a lot of woodland vegetation
The other is to use bacterial strains to cultivate, but the requirements for cultivation conditions are extremely high, and it is difficult to cultivate and cultivate in the cold regions of northern my country.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Preparation of strains:

[0019] (1) First prepare the solid culture medium (PDA) for separating Polyporus umbilicalis, separate the tissues of the wild Polyporus umbellata and the planted Polyporus umbellata respectively, and select excellent hyphae without miscellaneous bacteria respectively, and hybridize the above two strains to prepare Become the first-class Polyporus mother species, and then make the fungus branch of Amygdalus aeruginosa.

[0020] (2) Prepare the secondary bacterial culture medium, use 15% Maifuzi, 84% broad-leaved sawdust, and 1% white sugar to make the secondary bacterial culture medium, add water and stir evenly, and make the moisture content of the medium reach 55% -60%, put it into a bottle, plug it with a cotton plug, and sterilize it under normal pressure for more than 8 hours, take it out of the pot to cool down the bottle, and when the temperature of the culture medium reaches 28°C, insert the first-grade strain of Polyporus chinensis ...

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Abstract

A strain cultivating method used for chuling, belong to microbes cultivation technology field, its method is: producing strain: grow wild chuling and planted chuling are carried out structure abstraction, allomixis, producing female chuling species which is first class, and cultivating bacterium branch of shoestring fungus, producing second order strain, producing third order strain. 2. Cultivation: preparing adjuvant and wow-bed, cultivating the cultivation strain of chuling in wow-bed,producing chuling sclerote by quiescence of hyphal body, at last generating chuling.

Description

technical field [0001] The invention belongs to the technical field of microorganism cultivation. Background technique [0002] Polyporus is a commonly used medicinal fungus, which can be mainly used as a raw material for treating tumor drugs. [0003] At present, wild Polyporus is difficult to collect, and it is widely used in the medicinal industry. There are two ways of artificial planting, one is to use sclerotia to cultivate, but it takes 4-5 years to harvest, and the survival rate of cultivation is only about 60%, which consumes a lot of wood and is difficult to manage. Lots of woodland vegetation. The other is to use bacterial strains to cultivate, but the requirements for cultivation conditions are extremely high, and it is difficult to cultivate and cultivate in the cold regions of northern my country. Contents of the invention [0004] The purpose of the present invention is: [0005] The invention provides a method for cultivating Polyporus umbilicalis with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G1/04
Inventor 董庆忠
Owner 董庆忠
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