Super-critical flow extraction-efficient liquid-phase colour spectrum combined system
A high-performance liquid chromatography and supercritical fluid technology, applied in material separation, instruments, and analysis of materials, can solve the problems of large human error, cumbersome operation, and long time, and achieve small human error, avoid offline operation, and reduce sample volume. small effect
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[0029] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings.
[0030] Supercritical fluid extraction system: Jasco TM LC-1500 supercritical fluid extraction device (Jasco, Japan), including PU-1580 type CO 2 Pump, PU-2080plus liquid phase pump, CO-2060 thermostat, BP-1580-81 back pressure controller. Trap column: Hypersil C18 (4.6×150mm, 5μm) chromatographic column; analytical column: Hypersil C18 (4.6×250mm, 5μm) chromatographic column.
[0031] Liquid chromatography separation and detection system: Elite P230 high performance liquid chromatography (Elite, Dalian); Jasco UV-2075plus ultraviolet detector (Jasco, Japan); acetonitrile is supragradient chromatographically pure (Scharlau Chemie S.A., Barcelona, Spain), formic acid is analytically pure (Harbin Chemical Reagent Factory); mobile phase: A deionized water (1% formic acid) B acetonitrile (1% formic acid); linear gradient elution program: 0 ~ 60mi...
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