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Super-critical flow extraction-efficient liquid-phase colour spectrum combined system

A high-performance liquid chromatography and supercritical fluid technology, applied in material separation, instruments, and analysis of materials, can solve the problems of large human error, cumbersome operation, and long time, and achieve small human error, avoid offline operation, and reduce sample volume. small effect

Inactive Publication Date: 2007-03-28
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Application Information

AI Technical Summary

Benefits of technology

This patented technology solves problems with current methods that require complicated steps or multiple instruments during sampling processes due to potential contaminations from previous procedures. These technical features include efficient preparative processing without refrigerated storage, rapid analyte measurement while reducing operator interference, effective handling of large amounts of liquid samples at once, compact design, ease of use, and lower costs compared to existing techniques such as gas chromatography mass spectrometry. Overall, this new method provides faster results than older methods but also offers greater flexibility when analyzing different types of materials like liquids, gases, proteins, nucleic acids, etc., which are important tools for researchers working on various fields including healthcare science.

Problems solved by technology

Technological Problem: Overall, current techniques have limitations when trying to use biomolecules found naturally or synthetically due to their complexity and potential hazards associated therewith during analysis processes like ultraviolet light absorption spectrometry (UVAS)).

Method used

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  • Super-critical flow extraction-efficient liquid-phase colour spectrum combined system
  • Super-critical flow extraction-efficient liquid-phase colour spectrum combined system

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Embodiment Construction

[0029] The present invention will be described in further detail below in conjunction with the embodiments and accompanying drawings.

[0030] Supercritical fluid extraction system: Jasco TM LC-1500 supercritical fluid extraction device (Jasco, Japan), including PU-1580 type CO 2 Pump, PU-2080plus liquid phase pump, CO-2060 thermostat, BP-1580-81 back pressure controller. Trap column: Hypersil C18 (4.6×150mm, 5μm) chromatographic column; analytical column: Hypersil C18 (4.6×250mm, 5μm) chromatographic column.

[0031] Liquid chromatography separation and detection system: Elite P230 high performance liquid chromatography (Elite, Dalian); Jasco UV-2075plus ultraviolet detector (Jasco, Japan); acetonitrile is supragradient chromatographically pure (Scharlau Chemie S.A., Barcelona, Spain), formic acid is analytically pure (Harbin Chemical Reagent Factory); mobile phase: A deionized water (1% formic acid) B acetonitrile (1% formic acid); linear gradient elution program: 0 ~ 60mi...

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Abstract

This invention relates to supercritical fluid extraction devices and the HPLC. Specifically it is a supercritical fluid extraction-liquid chromatography system, including supercritical fluid extraction devices, the first three-way valve, the second three-way valve, trapping column, HPLC pump, analysis column and detector. Supercritical fluid extraction device connects with an interface of the first three-way valve, and the other two interfaces of the first three-way valve connect with the inlet of trapping column and liquid chromatography pump. Trap-export terminal connects with one interface of the second three-way valve, the other two import terminal interfaces of second three-way valve connect with the analytical column inlet and the associated waste water bottles, analytical column export terminal links to detector. This invention uses supercritical fluid extraction device to achieve sample active ingredient extraction, on-line analysis, avoiding the sample contamination and deterioration.

Description

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Claims

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Application Information

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Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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