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Anti-locust bacillus thuringiensis and preparation method for Bt locust killing agent

The technology of Bacillus aureus and thuringiensis spore is applied in the field of preparation of locusticide to achieve the effects of reducing pollution, low production and use costs, and reducing pesticide control costs

Inactive Publication Date: 2006-12-13
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0006] But up to now, relevant highly efficient anti-locust bacillus thuringiensis (Bt) specific bacterial strain and insecticidal crystal protein gene and Bt locust killer have not yet been reported

Method used

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  • Anti-locust bacillus thuringiensis and preparation method for Bt locust killing agent

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Embodiment Construction

[0041] 1. The preparation method of locust-resistant Bacillus thuringiensis bacterial strain BTH-13:

[0042] Utilizing the characteristics of spores that can withstand high temperatures of 80°C, at 80°C high temperature, the common solid bacterial culture medium is poured on the plate by the soil method, and cultured at 30°C for 3 days to isolate a single bacterial colony. The composition of the common solid bacterial culture medium is: 1% peptone, 0.5% beef extract, 0.5% sodium chloride, 1.5% agar, and the volume is supplemented with water to 100ml. A single colony smear was randomly selected, stained with carbofuran and observed under an optical microscope to screen for Bacillus thuringiensis that could produce spores and crystals. According to the first letter of the name of the soil sample source (Heilongjiang) where the Bt strain was isolated, its code BTH was formed, and because the strain was screened out from multiple strains of Bacillus thuringiensis isolated from th...

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Abstract

The invention discloses a grasshopper-resistant Bacillus thuringiensis BTH-13, CCTCC NO: M206061. The steps for preparing insecticide include: first shaking flask for fermentation, adding amidon, soya bean cake powder, cotton cake powder, protease leather, yeast powder, maize milk, magnesium sulfate heptahydrate, monobasic potassium phosphate, dibasic potassium phosphate, calcium carbonate alpha-amylase and make-up water by ratio in culture medium with 100 unit volumes; secondarily filling 100ml culture mediums in triangular bottle, then seeding BTH-13 after disinfection and culturing them until the brood-cell pulls off under finite temperature; third adopting the selected culture medium and fermenting them in full-automatic fermentation tank, acidifying the fermentation liquor after the fermentation, adding the emulsifier, and directly performing spray-drying after the compression of the fermentation liquor to prepare the wettable powder. The invention is characterized in that it is of strong specificity, is no fragmentation for insect natural enemy, is nontoxic for the people and the livestock and no pollution for environment, and can increase the agronomic crop output and has good application foreground.

Description

technical field [0001] The invention relates to a locust-resistant bacillus thuringiensis, and also relates to a preparation method of a locusticide. The locust-resistant bacillus thuringiensis is active and toxic to orthoptera migratory locusts and the like, and its active substance is insecticidal crystal protein. The locusticide prepared by the bacterial strain can be used for the control of orthoptera pests such as locusts, and the insecticidal crystal protein gene can be used for the construction of high-efficiency engineering bacteria and the research of insect-resistant transgenic plants. Background technique [0002] Bacillus thuringiensis (Bt) has played an important role in the biological control of pests because of its good insecticidal effect, strong specificity, convenient production and use, and no environmental pollution. Different Bt strains produce different insecticidal crystal proteins (Insecticidal Crystal Proteins, ICPs for short), w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01N25/12A01P17/00C12R1/08
Inventor 高梅影戴顺英宋玲莉
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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