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Kit for simultaneously detecting nonyl phenol, atrazine and estradiol and preparation method thereof

A technology of atrazine and nonylphenol, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that it is difficult to meet multiple detections, improve the inability to perform multiple detections at the same time, improve the cumbersome processing process, and save detection cost effect

Inactive Publication Date: 2006-11-22
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these techniques can only detect one or one type of pollutants at a time, and the pollutants, especially those in food, are unknown, and sometimes a variety of them coexist, so the above methods are difficult to meet the needs of multiple detection

Method used

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  • Kit for simultaneously detecting nonyl phenol, atrazine and estradiol and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Preparation of the immune chip:

[0056] (1) Prepare a glass slide with an aldehyde-based surface;

[0057] (2) Prepare the solution: ① dilute nonylphenol monoclonal antibody, atrazine monoclonal antibody and estradiol monoclonal antibody with phosphate buffer solution with pH of 7.0, so that the concentration of each antibody is 0.01mg / ml ; ② preparing a 2% bovine serum albumin solution by mass percentage with a phosphate buffer solution with a pH of 7.0;

[0058] (3) Immobilization of antibodies: use a spotting instrument to spot the solution prepared in step (2) step ① on different areas of the surface of the glass slide with an aldehyde-based surface, and place it at 37° C. for 3 hours at saturated humidity , rinsed with pH 7.0 phosphate buffer, rinsed with distilled water, and dried;

[0059] (4) Sealing of the glass slide: 10 μl of the bovine serum albumin solution prepared in step (2) in the step (2) was added dropwise to each area on the glass slide treated in...

Embodiment 2

[0061] Preparation of the immune chip:

[0062] (1) Prepare a glass slide with an aldehyde-based surface;

[0063] (2) Prepare the solution: ① dilute nonylphenol monoclonal antibody, atrazine monoclonal antibody and estradiol monoclonal antibody with phosphate buffer solution with pH 8.0 respectively, so that the concentration of each antibody is 1.0mg / ml ; ② preparing a bovine serum albumin solution with a mass percentage of 3% with a phosphate buffer solution having a pH of 8.0;

[0064] (3) Immobilization of antibodies: spotting the solution prepared in step (1) of step (2) on different areas of the surface of the glass slide with an aldehyde-based surface with a spotting instrument, and placed at 36°C and saturated humidity for 4 hours , rinsed with pH 8.0 phosphate buffer, rinsed with distilled water, and dried;

[0065] (4) Sealing of the glass slide: 10 μl of the bovine serum albumin solution prepared in step (2) in the step (2) was added dropwise to each area on the ...

Embodiment 3

[0067] Preparation of the immune chip:

[0068] (1) Prepare a glass slide with an aldehyde-based surface;

[0069] (2) Prepare the solution: ① dilute nonylphenol monoclonal antibody, atrazine monoclonal antibody and estradiol monoclonal antibody with phosphate buffer solution with pH 5.7, so that the concentration of each antibody is 0.5mg / ml ; ② preparing a 2% bovine serum albumin solution by mass percentage with a phosphate buffer solution with a pH of 5.7;

[0070] (3) Immobilization of antibodies: Spot the solution prepared in step (1) of step (2) on different areas of the surface of the glass slide with an aldehyde-based surface with a spotting instrument, and place it at 38° C. and saturated humidity for 0.5 hours , rinsed with pH 5.7 phosphate buffer, rinsed with distilled water, and dried;

[0071] (4) Sealing of the glass slide: 10 μl of the bovine serum albumin solution prepared in step (2) in the step (2) was added dropwise to each area on the glass slide treated ...

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Abstract

The disclosed reagent box for cholera morbus, atrazine and estradiol comprises: an immune chip as a slide connected to antibody for former product and closed by BSA, and special reagents for former products prepared by chemical derived, coupling and Cy3 labeling. This invention reduces cost, and improves defects in prior art.

Description

technical field [0001] The invention relates to a kit and a preparation method, in particular to a kit and a preparation method for simultaneously detecting nonylphenol, atrazine and estradiol. Background technique [0002] Biological immune chip refers to a microarray of high-density antigens or antibodies coated on a solid phase carrier, and is a new type of biological chip after the gene chip. The immune chip is composed of antigen or antibody microarrays immobilized on different types of support media. The position and composition of the immobilized molecules in the array are known. The antigen reacts with the recognition molecules on the chip, and then is detected by a specific scanning device, and the results are analyzed and processed by a computer. Compared with traditional detection technology, immunochip has the advantages of high throughput, automation, high sensitivity and multivariate analysis, and only requires a small amount of reagents and samples for each d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/532
Inventor 高志贤王涛
Owner INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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