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Nano sensor for detecting phenolic substance and its detecting method

A nano-sensor, sensor technology, applied in biochemical equipment and methods, microbial determination/inspection, color/spectral characteristic measurement, etc., can solve the problems of high cost, complicated operation, inability to realize real-time, online measurement of microbial degradation process, etc.

Inactive Publication Date: 2006-08-23
HUNAN UNIV
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Problems solved by technology

However, due to the complex components in the fermentation environment, commonly used detection methods for phenolic substances, such as spectrophotometry, are often interfered by light-interfering substances and turbidity in the solution, while high-performance liquid chromatography and other methods are complex in operation and high in cost, and cannot achieve microbial detection. Real-time, online measurement of degradation process

Method used

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  • Nano sensor for detecting phenolic substance and its detecting method
  • Nano sensor for detecting phenolic substance and its detecting method
  • Nano sensor for detecting phenolic substance and its detecting method

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Embodiment Construction

[0032] 1. Strain culture

[0033] Phanerochaete chrysosporium preheated and activated for 24 hours at 30°C was inoculated into PDA medium, and cultured statically at 37°C for 4 days for the amplification of the bacteria. The spores were inoculated into 25mL Kirk liquid medium in 8 250mL Erlenmeyer flasks, and cultured statically at 37°C for 2 days. The medium in each of the 4 Erlenmeyer flasks was mixed, homogeneously stirred for 20s, poured into a 250mL Erlenmeyer flask (100mL / flask), and shaken at 28°C for 63h at a speed of 150rpm.

[0034] The culture medium was filtered to obtain the fermentation broth.

[0035] Composition of PDA medium: 40 grams of potatoes, 4 g of agar, 4 g of glucose, and 200 mL of water.

[0036] Kirk liquid medium composition: glucose 20g / L, ammonium tartrate 0.22g / L, Tween 801g / L, benzyl alcohol 0.54g / L, KH 2 PO 4 2.56g / L, MgSO 4 ·7H 2 O1.46g / L, VB1 0.001g / L, succinic acid 1.18g / L, trace element solution 70mL / L; adjust to final pH4.5 with 0.1M...

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Abstract

This invention relates to a kind of nm sensor and a method for testing the phenol concentration in the Roles of phanerochaete chrysosporium aerobic broth, which takes Au nm particles fixed on the surface of a sensor as the crystal seeds, utilizes the theory of catalyst increase of Au nm particles under the action of phenol recovery and the rule of absorption spectrum variance to compute the variety of the nm particle absorption peak value in the spectrum to test the phenol concentration. This invention takes the hydroquinol concentration as the total phenol level to be analyzed by ultraviolet-visual absorption spectrum and applies a scan mirror to express the surface variance of the sensor, in which, the linear sphere of the hydroquinol concentration is 7x10< -6 >-3.81x10< -4 >M and the test limit is 7x10< -6 >M.

Description

technical field [0001] The invention relates to a method for detecting phenols, in particular to a nanometer sensor and a method for detecting phenols. Background technique [0002] Phenolic compounds are a very important class of basic raw materials in the organic chemical industry. They are widely used in many industrial productions such as petrochemicals, synthetic fibers, medicines, and pesticides. With the rapid development of the chemical industry and the improvement of people's living standards , a large number of phenolic compounds often enter the environment as direct industrial pollutants or transformed indirect pollutants, becoming the main pollutants in the environment. Phenolic compounds are toxic to organisms even at low concentrations, and the toxicity and biodegradability of many substituted phenols increase significantly with the increase of substituents, seriously threatening the ecological environment and human health. [0003] In recent years, the use of...

Claims

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Application Information

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IPC IPC(8): G01N21/31C12Q1/00
Inventor 汤琳曾光明牛承岗沈国励杨朝晖
Owner HUNAN UNIV
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