Sand-fixing water-keeping sprout-protecting bacteria preparation of low nutrient tolerance
A nutritional and bacterial technology, applied in the direction of bacteria, biochemical equipment and methods, applications, etc., can solve the problems of pure chemical and physical crust instability, no ecological significance, easy to be destroyed, etc.
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Embodiment 1
[0014] Preparation of liquid culture medium for bacteria resistant to low nutrients:
[0015] Potassium dihydrogen phosphate 0.01%, magnesium sulfate 0.01%, sodium chloride 0.02%, calcium sulfate 0.005%, calcium carbonate 0.2%, carbon 1mg / L, the balance is water, make culture medium according to conventional methods, pH7.0 , wherein the carbon source is glucose;
[0016] The isolated and purified two strains known to be resistant to low nutrition and produce extracellular mucopolysaccharides, Bacillus lentus and Beijienchia derxii, were equally inoculated in the prepared liquid medium, Cultivate at 15°C for 48 hours to prepare the culture stock solution of low-nutrition-resistant bacteria, and then dilute the original culture solution to 25%-75% with water; the components of extracellular mucopolysaccharide produced by low-nutrition-resistant bacteria: constituent cells Among the monosaccharides of exopolysaccharides, glucose, mannose and a glucose isomer are the main ones, f...
Embodiment 2
[0020] Preparation of liquid culture medium for bacteria resistant to low nutrients:
[0021] Potassium dihydrogen phosphate 0.01%, magnesium sulfate 0.01%, sodium chloride 0.02%, calcium sulfate 0.005%, calcium carbonate 0.2%, carbon 10mg / L, the balance is water, make culture medium according to conventional methods, pH7.1 , wherein the carbon source is mannitol;
[0022] The isolated and purified 2 strains known to be resistant to low nutrition and produce extracellular mucopolysaccharides, Bacillus lentus and Beijienchia derxii, were equally inoculated into the prepared liquid medium, Cultivate at 30°C for 60 hours to form the culture stock solution of low-nutrition-resistant bacteria, and then dilute the original culture solution to 25%-75% with water; among them, the components of extracellular mucopolysaccharide produced by low-nutrition-resistant bacteria: constituent cells Among the monosaccharides of exopolysaccharides, glucose, mannose and a glucose isomer are the m...
Embodiment 3
[0026] Preparation of liquid culture medium for bacteria resistant to low nutrients:
[0027] Potassium dihydrogen phosphate 0.01%, magnesium sulfate 0.01%, sodium chloride 0.02%, calcium sulfate 0.005%, calcium carbonate 0.2%, carbon 15mg / L, the balance is water, make culture medium according to conventional methods, pH7.2 , wherein the carbon source is glucose;
[0028] The isolated and purified 2 strains known to be resistant to low nutrition and produce extracellular mucopolysaccharides, Bacillus lentus and Beijienchia derxii, were equally inoculated into the prepared liquid medium, Cultivate at 50°C for 72 hours to prepare the culture stock solution of low-nutrition-resistant bacteria, and then dilute the original culture solution to 25%-75% with water; the components of extracellular mucopolysaccharide produced by low-nutrition-resistant bacteria: constituent cells Among the monosaccharides of exopolysaccharides, glucose, mannose and a glucose isomer are the main ones, ...
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