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Bacterial colony number sample for verifying microbiological capacity of food and its prepn process

A technology for microbiology and proficiency testing, which is applied in the field of microbiology proficiency testing samples, and can solve problems such as large differences in test results and uncertainty

Inactive Publication Date: 2005-12-14
卢行安 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since there is no standard substance in the total number of bacterial colony detection items, this results in randomness and uncertainty in the total number of bacterial colony inspections, and the test results vary greatly among laboratories. People pay special attention to food safety and hygiene, and food hygiene indicators are especially important The detection of microorganisms is particularly important. At the same time, the international mutual recognition of test results, the requirements of laboratory accreditation and the requirements of food trade make international food microbiology proficiency testing have special significance.

Method used

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  • Bacterial colony number sample for verifying microbiological capacity of food and its prepn process
  • Bacterial colony number sample for verifying microbiological capacity of food and its prepn process
  • Bacterial colony number sample for verifying microbiological capacity of food and its prepn process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] a strain recovery and subculture process

[0030] All standard strains are purchased from institutions designated by the government, and a strain certificate is attached to ensure the traceability of the strains. The standard strains were added to the nutrient broth, and the temperature was maintained at 36°C to revive and grow, and the revived strains were identified.

[0031] b enrichment process

[0032] After resuscitation, a single live bacterial strain grows in a nutrient-rich broth, and when it grows to a certain extent, it is centrifuged to obtain a sludge containing the corresponding single bacterial species.

[0033] The dominant bacterium Bacillus cereus was cultured to the stationary phase, and the other background bacteria were cultured to the end of the logarithmic growth phase.

[0034] C bacteria suspension preparation process

[0035] Mix the sludge obtained in the previous process with the substrate, the concentration of the substrate is: 4.8% treha...

Embodiment 2

[0051] According to the same preparation method as in Example 1, prepare food microbiology total bacterial colony proficiency verification samples, wherein, the strain culture medium is selected from nutrient agar slant medium, the matrix samples are the same, and the leading bacteria in the sample are 8 × 10 4 cfu / recombinant sample, background bacteria 2×10 3 cfu / recombined sample, and finally a proficiency test sample after homogeneity and stability tests.

Embodiment 3

[0053] According to the same preparation method as in Example 1, prepare food microbiology total bacterial colony proficiency verification samples, wherein, the bacterial strain culture medium selects the nutrient agar slant medium, the matrix samples are the same, and the leading bacteria in the sample are 5 × 10 4 cfu / recombinant sample, background bacteria 6×10 3 cfu / recombined sample, and finally a proficiency test sample after homogeneity and stability tests.

[0054] Gained sample homogeneity and stability detection method and result are as follows:

[0055] 16 samples were randomly selected, and the AOAC 990.12 total number of colony test method was used to test the total number of colonies of 2×16 samples under repeated conditions. The resulting data were statistically processed by one-way analysis of variance (ANOV), and the statistical steps and results were as follows:

[0056] ANOVA formula

[0057]

[0058] C = ...

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Abstract

The bacterial colony number sample for verifying food microbiological capacity relates to quality control technology in microbiological detection. The sample is matrix with added Bacillus cereus as leading bacteria and background bacteria. The matrix is practical food, and the background bacteria include one or several kinds of serratia marcescens, Citrobacter freundii, enteroaerogen and escherichia coli. The sample of the present invention has homogeneous colony number content, high finishing rate, and high stability, and may be used in test capacity verification of domestic and international labs. In the same time, the capacity verifying sample may be used in verification of detection method, calibration of test instrument, quality control and check of test result and other aspects.

Description

Technical field: [0001] The invention relates to the field of quality control in microbiological detection, in particular to a microbiological ability verification sample and a preparation method for the sample. Background technique: [0002] Under the trend of WTO and APEC committed to international trade liberalization, the traceability and comparability of test results have attracted more and more attention. In recent years, due to the attention of governments of various countries and the growth of market demand, the scale and field of international proficiency testing activities have developed rapidly, and proficiency testing has been widely used in government supervision and management, laboratory accreditation, and industrial product quality management. . Proficiency testing has become an important condition for international mutual recognition of laboratories. On the other hand, with the improvement of the degree of global economic integration, proficiency testing t...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 卢行安曹志军郑江秦成王刚卫锋陈明生
Owner 卢行安
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