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Central body located gene and its use in medicinal region

A genetic and medical technology, applied in medical preparations containing active ingredients, gene therapy, pharmaceutical formulas, etc.

Inactive Publication Date: 2005-08-24
RADIOLOGY INST ACAD OF MILITARY MEDICINE SCI PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the 20th century, with the rapid development of basic medicine, biotechnology and clinical therapeutics, some gratifying progress has been made in the prevention and treatment of hematopoietic disorders and malignant tumors. However, due to the reconstruction of hematopoietic function, malignant tumors Occurrence, development and transfer are extremely complex processes, so there are still many difficult problems to be overcome

Method used

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  • Central body located gene and its use in medicinal region
  • Central body located gene and its use in medicinal region
  • Central body located gene and its use in medicinal region

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Obtaining of cep gene cDNA

[0048] After RT-PCR reaction, two cDNAs of cep-11 (encoding 99 amino acids, molecular weight 11kDa) and cep-16 (encoding 142 amino acids, molecular weight 16kDa) were obtained

[0049] The RT-PCR reaction system is shown in Figure 10

Embodiment 2

[0050] Example 2 Northern Blotting and Dot Blotting Experiments

[0051] Material:

[0052] Hybridization membrane: Human RNA Master Blot TM Membrane, Human 12-Lane Multiple TissueNorthern(MTN) TM Blot membrane, Human Immune System Multiple Tissue Northern(MTN) TM Blot II membrane. All three membranes pre-transferred with RNA were purchased from CLONTECH. Human RNA Master Blot TM The name and distribution of samples on the membrane are as follows:

[0053] 1

2

3

4

5

6

7

8

A

whole brain

tonsils

caudate nucleus

cerebellum

cerebral cortex

layer

front lobe

seahorse

medulla

B

Occipital

putamen

substantia nigra

Temporal lobe

Thalamus

Dependent nucleus

spinal cord

C

the heart

aorta

skeletal muscle

intestin...

Embodiment 3

[0061] Example 3 Prokaryotic expression of Cep gene, preparation and purification of polyclonal antibody

[0062] 1. Material method

[0063] 1. Prokaryotic expression materials of Cep-11 and Cep-16: pGEX-4T-2 vector, GST fusion expression protein purification system (Bulk and Redpack GSTPurification Modules, MicroSpin GST Purification Module), Anti-GSTAntibody and ECL chromogen were purchased from Amersham Pharmacia.

[0064] method:

[0065] A. Construction of prokaryotic expression vector

[0066] Full-length Cep-11 and Cep-16 were inserted into the pGEX-4T-2 vector with EcoR 1 and Xho 1 restriction sites, respectively, to form pGEX-4T-2-Cep-11 and pGEX-4T-2-Cep-16 recombinant vectors . Then, strain JM109 was transformed for expression.

[0067] B. Expression and purification of GST-Cep-11 and GST-Cep-16 fusion proteins

[0068] The expression strain JM109 was transformed with pGEX-4T-2-GST-Cep-11 and pGEX-4T-2-Cep-16. Pick positive clones and shake overnight at 37°C...

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Abstract

A centrosome located gene cep is disclosed, which is prepared through sequencing, biological information analysis, and gene technique. It can be used as the candidate gene for treating the diseases is hemopoietic system and the generation and transfer of tumor, or as the target point of genetically engineered medicine.

Description

technical field [0001] The present invention relates to a gene, in particular to a novel centrosome-localized gene, and also relates to the application of the gene in the field of medicine. Background technique [0002] The prevention and treatment of hematopoietic disorders and malignant tumors has always been an international research hotspot. In the 20th century, with the rapid development of basic medicine, biotechnology and clinical therapeutics, some gratifying progress has been made in the prevention and treatment of hematopoietic disorders and malignant tumors. However, due to the reconstruction of hematopoietic function, malignant tumors The occurrence, development and transfer of the disease are extremely complicated processes, so there are still many difficult problems to be overcome so far. One of the important reasons for this is that many important functional genes directly involved in these processes and their molecular regulatory networks have not yet been c...

Claims

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Application Information

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IPC IPC(8): A61K31/7088A61K38/16A61K48/00A61P7/00A61P35/00C07H21/04
Inventor 贺福初王兆卿朱云平鱼咏涛吴松锋魏汉东邢桂春
Owner RADIOLOGY INST ACAD OF MILITARY MEDICINE SCI PLA
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