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Antibodies against fibroblast growth factor 23

A fibroblast, growth factor technology, applied in the direction of fibroblast growth factor, anti-growth factor immunoglobulin, growth factor/inducing factor, etc.

Inactive Publication Date: 2005-07-13
KYOWA HAKKO KIRIN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no substance that selectively inhibits or removes FGF-23 using the above method has been found.

Method used

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  • Antibodies against fibroblast growth factor 23
  • Antibodies against fibroblast growth factor 23
  • Antibodies against fibroblast growth factor 23

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0082] Figure 1B shows the full-length FGF-23 protein, N-terminal fragment and C-terminal fragment purified from the supernatant of CHO-FGF-23 cell culture, cell culture supernatant of CHO-FGF-23 The measurement results obtained after separation by SDS-polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue (CBB).

[0083] Figure 1C shows the assay results of a recombinant FGF-23 protein, a recombinant FGF-23RQ protein, and its metabolites by Western blotting using an anti-FGF 23-148 antibody, this anti-FGF 23- 148 antibody was obtained by separating the culture supernatants of CHO-FGF-23H cells and CHO-FGF-23RQ cells, and then separating the cells by SDS-polyacrylamide gel electrophoresis. (Example 5)

Embodiment 5

[0084] Figure 2 shows the detection of FGF-23H protein by sandwich ELISA using a hFGF23-25 ​​antibody as an immobilized antibody and a hFGF23-237 as a detection antibody (this detection antibody is a polyclonal antibody against FGF-23). The result obtained after performing the measurement.

Embodiment 7

[0086] Figure 3 shows the results of immunoprecipitation of FGF-23H protein with anti-FGF-23 monoclonal antibody, separation of the protein by SDS-polyacrylamide gel electrophoresis, and detection of the protein with 2A2B antibody and 1C3H antibody. (Example 10)

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Abstract

The purpose of the present invention is to provide an antibody against fibroblast growth factor 23. The antibody is obtained by immunizing an animal with a polypeptide which comprises an amino acid sequence represented by SEQ ID NO: 1, or an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of 1 or several amino acids, and has fibroblast growth factor-23 activity and activity to control phosphate metabolism or vitamin D metabolism, and is shown by the following (a), (b), or (c): (a) an antibody, which recognizes an amino acid sequence between the 180 and the 194, or the 237 and the 251<st> amino acid residues represented by SEQ ID NO: 1; (b) an antibody, which is produced by a hybridoma whose accession number is FERM BP-7838, FERM BP-7839, FERM BP-7840, or FERM BP-8268; or (c) an antibody, which is competitive with the antibody produced by the hybridoma whose accession number is FERM BP-7838, FERM BP-7839, FERM BP-7840, or FERM BP-8268 upon binding with the polypeptide comprising the amino acid sequence represented by SEQ ID NO: 1. <IMAGE>

Description

technical field [0001] The present invention relates to antibodies against fibroblast growth factor-23 (FGF-23). [0002] More specifically, the present invention relates to antibodies that provide methods for diagnosing diseases or pathological conditions associated with accumulation or reduction of FGF-23 protein in vivo by appropriately detecting and measuring FGF-23, said method being capable of suppressing FGF -23 to improve or treat the symptoms or pathological state of the disease caused by the excessive effect of FGF-23, the present invention also relates to a method for preparing an antibody and a method for using the antibody. Background technique [0003] Fibroblast growth factor was purified for the first time from calf pituitary as a stimulating substance of the fibroblast cell line NIH3T3. Then, similar proteins were identified in various tissues, and a group of these substances formed a polypeptide family (FGF family). To date, 22 types of proteins belonging...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61P19/08C07K14/50C07K16/22C12P21/08
CPCA61K39/00A61K2039/505C07K14/50C07K16/22A61P13/00A61P13/12A61P17/04A61P19/00A61P19/08A61P19/10A61P21/00A61P3/00A61P3/12A61P3/14A61P35/00A61P5/06A61P5/18A61P5/20A61P7/08C07K16/18A61K39/395G01N33/53
Inventor 山崎雄司岛田考志山下武美
Owner KYOWA HAKKO KIRIN CO LTD
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