Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Process for raising producing rate of taxad alcohol of Taxus chinensis endogeny fungus fermenting material

A technology of endophytic fungi and paclitaxel, which is applied in the direction of fermentation and fungi, and can solve the problem of low fermentation yield

Inactive Publication Date: 2005-06-08
SOUTHWEST UNIVERSITY
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These fermentation production levels are very low, and

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]In this example, two strains of endophytic fungi XT1 (high-yielding paclitaxel synthetic precursors 10-desacetylbaccatin III and baccatin III, but almost no paclitaxel) and XT5 (taxol-producing ) cultures were mixed to produce paclitaxel.

[0035] The identification of the two endophytic strains involved in this example (Fungus Identification Handbook, Wei Jingchao's posthumous work, Shanghai Science and Technology Press, 1979, 645-649) is as follows:

[0036] bacteria

code name

host plant

product

Morphological characteristics

Classification status

XT1

Southern yew

Taxus chinnsis

var.mairei

10-to B

Acyl baccatin

III and Bar

Catin III

Only sterile hyphae, no sexual and

Asexual spores, mycelium in the form of cotton wool,

No sclerotium was found, the hyphae were thick and intertwined into

Reticular, the fungus is white in the early stage, and in the later stag...

Embodiment 2

[0057] In Example 1, the taxol-producing endophytic fungus was replaced by the group Rhizoctonia (Phacodium Pers.ExWallr.); the high-yield paclitaxel synthetic precursor 10-deacetylbaccatin III and baccatin III were replaced by Steline and Phytophthora. (Ectostroma Fr.). In the fermentation medium, the sucrose was changed to 10g / L, the phenylalanine was changed to 1mg / L, and other conditions remained unchanged. The mixed culture of TAX-49 and TAX-25 was carried out, and the culture of TAX-49 and TAX-25 was carried out alone. According to the detection by high performance liquid chromatography, the contents of paclitaxel in the fermented product were: 842.5648μg / L, 231.5830μg / L, 0μg / L.

Embodiment 3

[0059] Change sucrose into 10g / L in the fermentation medium in example 1, change phenylalanine into 1mg / L, change sodium acetylsalicylate into 0.5mg / L, change oleic acid into 10ml / L, and other conditions are constant, XT5 and XT1 were mixed cultured, XT5 and XT1 were cultured alone, and the contents of paclitaxel in the fermented product were detected by high performance liquid chromatography: 1094.0274 μg / L, 276.7554 μg / L, and 0 μg / L, respectively.

[0060] instance number

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A method for increasing the output rate of taxol in the endofungus fermented substance of enqlist yew features that the endofungus generating taxol and the synthesized precursors 10-deacetyl Bakating C and Bakating C able to generate taxol are individually cultured, and the cultured substances are then mixed and cultured while additional culture medium is added. As a resultant the output rate of taxol is greatly increased.

Description

technical field [0001] The method utilizes paclitaxel-producing endophytic fungal culture and paclitaxel synthetic precursor-producing endophytic fungal culture to carry out mixed culture to greatly increase paclitaxel content in the fermented product, and belongs to the field of biotechnology. Background technique [0002] Since wani et al. extracted paclitaxel from the bark of Taxus brevifolia Nutt. and found that it has anticancer effects, researchers have successively discovered its unique anticancer mechanism and can treat various diseases. cancer. Therefore, people began to extract paclitaxel from the bark of Taxus in large quantities to meet the needs of the pharmaceutical market. Because the yew tree is a rare plant in the world, extracting paclitaxel from the bark will inevitably seriously damage the natural resources and endanger the ecological balance. In order to solve the shortage of drug sources, scholars at home and abroad have done...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/14C12P17/02
Inventor 谈锋胡凯祝顺琴唐克轩廖志华
Owner SOUTHWEST UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com