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Peptide library, its synthesis process and active segment screened from the peptide library

A library, amino acid technology, applied in the fields of peptide/protein components, chemical instruments and methods, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2005-04-27
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0037] At present, combinatorial chemistry techniques have been successfully used in the synthesis of small molecular substances in the field of compound synthesis, but there are no reports on the synthesis of macromolecular substances such as peptides.

Method used

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  • Peptide library, its synthesis process and active segment screened from the peptide library
  • Peptide library, its synthesis process and active segment screened from the peptide library
  • Peptide library, its synthesis process and active segment screened from the peptide library

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083]Design and synthesize the "cross-overlapping" sequence of each adjacent 10 amino acid sequences from 1 to 600 amino acid sequences of the Spike (S) protein, as shown in the table below. From right to left is the order of synthesis, representing the number of synthesis steps as Arabic numerals 1 to 10.

[0084] Table 2 Synthesized 600 sequences with a length of 10 amino acids

[0085] Number 10 9 8 7 6 5 4 3 2 1

[0086] 1) M F I F L L F L T L

[0087] 2) F I F L L F L T L T

[0088] 3) I F L L F L T L T S

[0089] 4) F L L F L T L T S G

[0090] 5) L L F L T L T S G S

[0091] 6) L F L T L T S G S D

[0092] 7) F L T L T S G S D L

[0093] 8) L T L T S G S D L D

[0094] 9) T L T S G S D L D R

[0095] 10) L T S G S D L D R C

[0096] 11) T S G S D L D R C T

[0097] 12) S G S D L D R C T T

[0098] 13) G S D L D R C T T F

[0099] 14) S D L D R C T T F D

[0100] 15) D L D R C T T F D D

[0101] 16) L D R C T T F D D V

[0102] 17) D R...

Embodiment 2

[0935] The "cross-overlapping" sequence of each 10-position sequence of amino acids 601 to 1246 of Spike (S) protein was designed and synthesized as follows. From right to left is the order of synthesis, representing the number of synthesis steps as Arabic numerals 1 to 10.

[0936] Table 14 The 646 sequences synthesized in the Spike(S) protein with a length of 10 amino acids

[0937] Number 10 9 8 7 6 5 4 3 2 1

[0938] 601) V N C T D V S T A I

[0939] 602) N C T D V S T A I H

[0940] 603) C T D V S T A I H A

[0941] 604) T D V S T A I H A D

[0942] 605) D V S T A I H A D Q

[0943] 606) V S T A I H A D Q L

[0944] 607) S T A I H A D Q L T

[0945] 608) T A I H A D Q L T P

[0946] 609) A I H A D Q L T P A

[0947] 610) I H A D Q L T P A W

[0948] 611) H A D Q L T P A W R

[0949] 612) A D Q L T P A W R I

[0950] 613) D Q L T P A W R I Y

[0951] 614) Q L T P A W R I Y S

[0952] 615) L T P A W R I Y S T

[0953] 616) T P A W R I Y S T G

[0954] 617) P...

Embodiment 3

[1831] The "cross-overlapping" sequence of every 10 amino acids in the entire amino acid sequence of Membrane (M) protein, Envelop (E) protein and Nucleocapside (N) protein was designed and synthesized as follows. From right to left is the order of synthesis, representing the number of synthesis steps as Arabic numerals 1 to 10.

[1832] M-protein:

[1833] Table 25 212 sequences of 10 amino acids in the synthesized M-protein

[1834] Number 10 9 8 7 6 5 4 3 2 1

[1835] 1. M A D N G T I T V E

[1836] 2. A D N G T I T V E E

[1837] 3. D N G T I T V E E L

[1838] 4. N G T I T V E E L K

[1839] 5. G T I T V E E L K Q

[1840] 6. T I T V E E L K Q L

[1841] 7. I T V E E L K Q L L

[1842] 8. T V E E L K Q L L E

[1843] 9. V E E L K Q L L E Q

[1844] 10. E E L K Q L L E Q W

[1845] 11. E L K Q L L E Q W N

[1846] 12. L K Q L L E Q W N L

[1847] 13. K Q L L E Q W N L V

[1848] 14. Q L L E Q W N L V I

[1849] 15. L L E Q W N L V I G

[1850] ...

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Abstract

The peptide library has its every peptide has the general expression of Z-XnXn+1X n+2X n+3X n+4X n+5X n+6X n+7X n+8X n+9-Y, whereX is one of L-configuration natural amino acid residue, n is the natural sequence position of Xn amino acid in the protein sequence and is integral, Y expresses NH2 or OH, and Z is H or acetyl radical Ac. The polypeptide in the said protein sequence is preferably coronavirus SARS-CoV related S, M, E and N protein polypeptide sequence. The present invention also relates to the amino acid sequence with SARS-CoV antigen determinant function, such as those shown in SEQ ID No. 1 to SEQ ID No. 112. The present invention also relates to one kind of 'mixing-separating' physical encoding method for synthesizing peptide library with at least 2-50 amino acid residues.

Description

Background technique [0001] Human-related coronavirus (Severe Acute Respiratory SyndromeCoronavirus (SARS-CoV)) has been identified as a new type of virus, and humans have determined its gene sequence at the fastest speed so far, mainly composed of RNA-dependent RNApolymerase, Spike (S ), Membrane (M), Envelope (E), Nucleocapside (N), Polymerase (P) and other protein components (1, Marra MA et al., The genome sequence of the SARS-associated Coronavirus, Scienceexpress, published online at www.scienceexpress.org , 1 May, 2003; 2. Rota PA et al., Characterization of a novel coronavirus associated with severe acute respiratory syndrome, Sciencexpress, published online at www.scienceexpress.org , 1 May, 2003; 3. Qin E'de, et al., A complete sequence and comparative analysis of aSARS-associated virus (Isolate BJ01), Chinese Science Bulletin 2003, 48(10): 941-948; 4. Peoros JS, et al., Coronavirus as a possible cause of severe acute respiratory syndrome, The Lancet, published onlin...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P11/00A61P31/14C07K14/165
Inventor 刘刚何维李莉崔莲仙寇宾宾胡洪波王占国郝志勇张亮李旭琴倪安平刘勇孙曼霁李前
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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