Biological total internal reflection type near-field scan microscope

Abstract

A biology inside total reflection near field scanning microscope is consisted of four parts. The first is excitation part comprising laser maser, collimation and reflector of optical system, the second is wave creation part comprising inverse trapezoidal prism and sample to be tested on its top surface, the third is collection, storage and disposal of information comprising optical fiber probe, driving scanning part and second photoelectric probe head and computer, and the fourth is fiber probe height controller comprising optical information colleting part and first photoelectric probe head, and the fourth one has the property of having a fluorescent dye macromolecule or enzyme or quantum point sticking to needle tip or near that of the fiber probe. Being a optical total reflection near field scanning microscope used in testing biological cell microstructure and single element, the invention can also uncover the discipline, which can create the most effective spatial resolution.

Description

technical field [0001] The invention relates to near-field scanning imaging optics, in particular to a biological total internal reflection near-field scanning microscope for biological research. Background technique [0002] In 1928, E.H.Synge first proposed the idea of ​​near-field scanning imaging, which opened up a new way of super-diffraction limit resolution imaging. In the 1970s, people used microwaves to realize the initial near-field optical scanning imaging, and it has a history of more than 30 years. Although the near-field scanning imaging technology has gradually matured, the resolution of near-field optical scanning microscopy has not been settled, and it is still improving until now. For example, the revelation of the spatial coherence phenomenon related to the distance of the object interface provides a theoretical basis for further improving the resolution, and the use of a lock-in amplifier to lock the vibration frequency of the probe in order to accuratel...

AI Technical Summary

Problems solved by technology

Although the near-field scanning imaging technology has gradually matured, the resolution of near-field optical scanning microscopy has not been conclusive, and it is still being improved until now.

[0004] (2) There is a lack of universally recognized theories to describe the imaging process and expected imaging results

There are many difficulties in detecting biological surface information. One is that the information obtained by the biological surface is low because of the low reflectivity of the biological surface; very low noise ratio

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