Paphiopedilum aseptic seeding and tissue culture technology

A technology of aseptic sowing and tissue culture, applied in the fields of botany equipment and methods, gardening, gardening tools/equipment, etc., can solve the problems of complex ploidy of hybrids, difficult to mass-produce, difficult asexual proliferation, etc., and achieve a simple formula. Effective, high germination rate and simple cultivation equipment

Inactive Publication Date: 2004-11-03
SOUTH CHINA PLANT INST CHINESE ACAD OF SCI
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new technique allows scientists to study plants more easily by growing them under controlled conditions without exposure during testing time. It also makes plantings faster and stronger than previous methods that were previously possible due to its simplicity and efficiency.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving paphodnaretea's ability for high yields during sexual multiplication without being affected with other crops that may have lower quality levels compared to non-target crop systems such as rice.

Method used

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Examples

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Embodiment

[0012] 1. Materials: When flowering, select a vigorous Paphiopedilum parent plant for artificial pollination. After pollination, the fruit matures within 120 days and is used as an explant for sowing.

[0013] 2. Aseptic sowing: remove the fruit, soak it in 75% alcohol for 30 seconds, put it in 0.1% mercuric acid solution for 20 minutes, rinse it with sterile water for 4-5 times, cut the fruit, and inoculate the white fruit with an inoculation needle. The powdered embryos were inoculated into the R medium supplemented with 0.5 mg / L 6-benzylpurine, 0.5 mg / L naphthalene acetic acid, 2 g / L activated carbon, 10% coconut milk and vitamin ingredients in NDM medium, and the embryos germinated in about 30 days After about 60 days, a complete plantlet is formed, and the plantlet is transferred to the improved R medium in time with the addition of 100 ml / liter of potato extract, 0.2 mg / liter of 6-benzylpurine, and 0.5 mg / liter of naphthaleneacetic acid.

[0014] 3. Tissue culture: cultu...

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Abstract

The present invention relates to the abacterial seeding and tissue culture of Paphiopedilum. The Dendrobium is one kind of national first order protected plant and is hard to germinate naturally owing to its incomplete development of embryo. The present invention provides technology of abacterial seeding and tissue culture of Paphiopedilum to obtain great amount of test tube seedling via utilizing artificial nutritious liquid and abacterial seeding, proliferate small seedlings and obtain complete plants via rooting culture. The said seedling growing technology has the advantages of high germination rate, fast seedling growing rate, high seedling quality, etc.

Description

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Claims

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Application Information

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Owner SOUTH CHINA PLANT INST CHINESE ACAD OF SCI
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