Blood vessel vitrifying storage liquid and blood vessel storage method
A technology of vitrification and preservation solution, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of high chemical toxicity of vascular cells, affecting vascular survival rate, unstable vascular cell survival rate, etc. High transplant survival rate, short freezing time and wide application range
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Embodiment 1
[0022] Example 1: Dilute Ficoll70 (the average relative molecular mass is 70,000) and trehalose into 30% and 17% mixed solutions (FT solution) with M199 solution, add 1% azone, and then dilute vinylglycerin with FT solution Alcohol and polyvinyl alcohol (20:1) are mixed and diluted to 30% (v / v) PEFT30 vitrification solution, and 0.002% of anionic surfactants such as sodium lauryl sulfate are added. At a room temperature of 20°C, transfer the constructed tissue engineered blood vessel or artificial blood vessel into a 50ml plastic tube or plastic bag containing PEFT30 vitrification solution to equilibrate for 1 minute, seal it and put it directly into liquid nitrogen for cryopreservation. When thawing is required, take the cryopreserved tube or bag out of the liquid nitrogen and immerse it directly in a 25°C water bath for about 20 seconds to complete the thawing procedure.
[0023] The above-mentioned thawed blood vessels were digested with enzymatic digestion method to digest...
Embodiment 2
[0024] Embodiment 2: Ficoll70 (average relative molecular mass is 70000) and trehalose (Trehalose) are diluted into 30% and 17% mixed solution (FT solution) with M199 liquid, then ethylene glycol and polyvinyl alcohol are respectively mixed with FT solution (30:1) mixed and diluted to 40% (v / v) PEFT40 vitrification solution, adding 0.8% azone and a small amount of anionic surfactants such as sodium lauryl sulfate. At a room temperature of 25°C, transfer the aseptically obtained homologous or heterologous blood vessels into a 50ml plastic tube or plastic bag containing PEFT40 vitrification solution to equilibrate for 1 minute, seal it and put it directly into liquid nitrogen for cryopreservation. When thawing is required, take the cryopreserved tube or bag out of the liquid nitrogen and immerse it directly in a 25°C water bath for about 20 seconds to complete the thawing procedure.
[0025]The above-mentioned thawed blood vessels were digested with enzyme digestion method to di...
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