Fragrant citrus iron chelating reductase gene

A technology of iron chelation and reductase, which is applied in the fields of oxidoreductase, genetic engineering, plant gene improvement, etc., can solve the problems such as the research report of iron chelation reductase gene, so as to promote the overall health level and improve iron content. Effect of nourishment, promotion improvement

Inactive Publication Date: 2004-03-17
SOUTHWEST AGRICULTURAL UNIVERSITY
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Problems solved by technology

Both Arabidopsis thaliana and pea are herbaceous plants. So far, there have been no research reports on iron chelation reductase genes in woody plants

Method used

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Embodiment

[0019] Using Ziyang orange as the material, after iron-deficiency induction and culture, the total RNA of induced roots was extracted, and the mRNA was obtained by isolation and purification. The cDNA was obtained by reverse transcription using the mRNA as a template. The degenerate primer 5'aagctgcagtggca(ct)cc(agct)tt(ct)ac3' was designed with the cDNA sequence of the iron chelating reductase FRO2, and the above cDNA was used as a template, using the YADE (Y-shaped Adaptor Dependent Extension) method to obtain The cDNA sequence of the iron chelating reductase gene of orange was subcloned into pGEM-T, and the iron chelating reductase gene cjFRO2 of orange was obtained. The cjFRO2 gene subcloned into the pGEM-T vector was digested with EcoRI and KpnI restriction enzymes to recover a 2376bp fragment, which was ligated with the pBS-SK vector after digestion with the same endonuclease, and transformed to obtain an intermediate vector pBSFRO2. Then the intermediate vector pBSFRO2 was ...

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Abstract

The Citrous junos Ferric-chelated Reductase gene CjFR02 is obtained with iron deficiency tolerant Ziyang citron as material and through one series of biotechnological process. The gene has cDNA sequence length of 2403 bp, contains one 2103 bp opened reading frame, encodes 710 amino acids, and has one 64 bp upstream non-coding sequence and 3' end polyA tail. The homology comparison results show that the cloned cDNA sequence has higher homology with reported Ferric-chelated Reductase gene FR02, 49-52 % of same amino acids and 68 % of similar amino acids. The gene engineering of applying the gene and its plant expression carrier in plant can improve the iron deficiency characteristics of grain crop, vegetable and fruit tree to produce high quality agricultural products capable of improving human iron nutrients and raising health level.

Description

Technical field [0001] The present invention relates to the field of biotechnology, in particular to an orange iron chelate reductase gene. Background technique [0002] According to statistics from the World Health Organization, more than 3 billion people in the world are at risk of iron deficiency (Goto, 1999). In our country, nutritional iron deficiency in infants and young children is very common, which directly threatens the overall health of the people. Plants are an important source of iron for humans, especially crops. The main form of iron absorption by plants is reduced iron (Fe 2+ ). However, iron is mainly in the oxidized state of iron (Fe 3+ ) Exists, reduced iron (Fe 2+ ) Is very limited, especially in alkaline or neutral soils. The percentage of soils with potential iron-deficiency plants in the world is as high as 25%-40% of the land area. In my country, from the Sichuan Basin to the Inner Mongolia grassland in the south, from the Huaibei Plain in the east to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/00C12N9/02C12N15/53
Inventor 李德谋肖月华侯磊罗小英周泽扬裴炎
Owner SOUTHWEST AGRICULTURAL UNIVERSITY
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