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Purified plyporus laccase and nucleic acid for coating the laccase

A technology of polyporous bacteria and laccase, which is applied in the fields of nucleic acid fragments of laccase, phenol oxidase and purified enzymes, and can solve problems such as low yield

Inactive Publication Date: 2004-03-03
NOVO NORDISK AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous attempts to express basidiomycete laccases in recombinant hosts resulted in extremely low yields

Method used

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  • Purified plyporus laccase and nucleic acid for coating the laccase
  • Purified plyporus laccase and nucleic acid for coating the laccase
  • Purified plyporus laccase and nucleic acid for coating the laccase

Examples

Experimental program
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Effect test

Embodiment I

[0052] Example I. Isolation of Polyporus pinsitus laccase

[0053] Materials and methods

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Abstract

The present invention relates to isolated nucleic acid constructs containing a sequence encoding a Polyporus laccase, and the laccase proteins encoded thereby.

Description

[0001] This application is a divisional application of the application No. 95194301.4 submitted on June 15, 1995, and the invention title is "purified Polyporus laccase and nucleic acid encoding the enzyme". technical field [0002] The present invention relates to isolated nucleic acid fragments encoding a fungal oxidoreductase, and purified enzymes derived from the fragments. More particularly, the present invention relates to nucleic acid fragments encoding a phenoloxidase, in particular a laccase from Basidiomycetes-Polypores. Background technique [0003] Laccases (benzenediol:oxygen oxidoreductases) are multi-copper enzymes that catalyze the oxidation of phenolics. Oxidation of suitable phenolic substrates by laccases leads to the production of aryloxy intermediates that undergo terminal polymerization to form a mixture of dimerization, oligomerization, and polymerization reaction products. This reaction actually plays an ...

Claims

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Application Information

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IPC IPC(8): C12N15/09A61K8/00A61K8/66A61Q5/10C09B53/02C12N1/15C12N9/02C12N15/00C12N15/53C12N15/80C12R1/645C12R1/66D06M16/00D06P1/00D06P1/32D21C5/00
CPCC12N9/0061D06M16/003D06P1/32A61K8/66D21C5/005D06P1/0048D06P1/0004C12N15/80A61Q5/10
Inventor D·S·亚夫尔F·许H·达尔伯吉P·施耐德D·A·阿斯林格
Owner NOVO NORDISK AS
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