Tissue culture reproduction method of snake grass
A technology for tissue culture and heliconia, applied in the field of culture medium, can solve problems such as no large-scale production and patent application, and achieve the effects of strong resistance, rapid emergence and high stability
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Embodiment 1
[0015] 1. Material: Rhizome of Heliconia rostrata Ruiz & Pavon.
[0016] 2. The method of explant induction material disinfection: excavate vigorously growing heliconia rhizomes from the field, remove the roots and leaves, rinse with tap water, and soak in 0.1% potassium permanganate solution for 5 minutes , take out the towel and dry the rhizomes, then soak them in 0.1% carbendazim solution for 5 minutes, pack them in a black plastic bag with a small amount of medicine, and place them in an incubator at about 30°C for dark cultivation. A 4-6 centimeter sprout can be formed, and the sprout is used as an explant for preliminary sterilization. Soak the explants in alcohol for 30 seconds on the ultra-clean workbench, then soak them in 0.1% mercury liter for 10 minutes, rinse them with sterile water for 3 times, and peel off the scales of the buds with a scalpel. For each layer of scales, use 0.1 Soak in % mercury chloride for 1 minute, rinse with sterile water three times until ...
Embodiment 2
[0023] Rainbow heliconia (H. psittacorum L.f.cv. Sassy) was used as the explant. The basic operation method is consistent with embodiment 1. But its adventitious bud induction medium is MS+6-benzylpurine 5 mg / L+naphthalene acetic acid 0.5 mg / L+15% coconut water; cluster bud proliferation medium is MS+6-benzylpurine 5 mg / L+ Naphthalene acetic acid 0.5 mg / L; strong seedling medium is MS+6-benzyl purine 1 mg / L + naphthalene acetic acid 0.1 mg / L. The survival rate of transplanted test-tube seedlings can reach 100%.
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