Transgenically produced decorin

Abstract

The invention provides transgenically produced decorin and methods of making and using transgenically produced decorin.

Description

[0001] This application claims the benefit of provisional application 60 / 201,932, filed May 5, 2000, the contents of which are hereby incorporated in their entirety. Background of the invention [0002] An increasing number of recombinant proteins are being developed for therapeutic, diagnostic, agricultural, veterinary, nutritional and other uses; however, many of these proteins can be difficult or expensive to produce in functional form in large quantities using conventional methods. [0003] Conventional methods often involve inserting the genes responsible for the production of specific proteins into host cells such as bacteria, yeast or mammalian cells. These cells are grown in culture medium and the protein of interest is recovered from the cells or culture medium. Traditional bacterial or yeast systems sometimes fail to produce complex proteins in functional form. Although certain mammalian cells can replicate complex proteins, they are often difficult or expensive to ...

AI Technical Summary

Problems solved by technology

Traditional bacterial or yeast systems sometimes fail to produce complex proteins in functional form

Although some mammalian cells can replicate complex proteins, they are often difficult or expensive to culture and produce only relatively low amounts of protein

In addition, non-secreted proteins are relatively difficult to purify from prokaryotic or mammalian cells since they are often not secreted into the culture medium