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Method of raising the yield of insect baculovirus with insect juvenoid

A technology of juvenile hormone and baculovirus, which is applied in biochemical equipment and methods, viruses/phages, microorganisms, etc., can solve the problems of increased silk protein synthesis, and achieve the effect of low cost and increased virus yield

Inactive Publication Date: 2003-03-19
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the fact that the treatment of silkworm and cerecium silkworm with JHA can prolong the larval stage, promote the synthesis of fat body protein and the replication of silk gland DNA, RNA transcription and protein translation, and increase the synthesis of silk protein, and what effect does JHA have on the proliferation of insect baculovirus? Research in this area has not yet been reported

Method used

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Examples

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Effect test

Embodiment 1

[0012] The present invention will be further described below through embodiment. Example 1 Effect of juvenile hormone analogues on beet armyworm larvae

[0013] Table 1 Effects of juvenile hormone analogues on beet armyworm larvae

[0014] Dosage of JHA treatment Larval survival rate Prolonging the time of larval stage

[0015] Insect number (head)

[0016] (μg / head) (%) (day)

[0017] Early 4th age 30 2 90.4 0

[0018] At the beginning of 4 years old 30 5 78.3 0

[0019] Early 4th age 30 10 56.7 0

[0020] 4 years old 30 2 94.2 0

[0021] 4 years old 30 5 84.9 0

[0022] 4 years old 30 10 62.3 0

[0023] At the end of the 4th age 30 2 98.1 0

[0024] At the end of the 4th age 30 5 92.0 1.5

[0025] At the end of 4th instar 30 10 86.5 2

[0026] Early 5th age 30 2 100 0

[0027] Early 5th age 30 5 100 1.7

[0028]Early 5th age 30 10 100 3

[0029] Table 1 shows the effects of treating beet armyworm larvae with diff...

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PUM

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Abstract

The present invention relates to the application of insect juvenoid analog in the proliferation of insect baculovirus, and is especially the method of raising the yield of insect baculovirus with insect juvenoid. The method includes treating effective insect larva in its initial end period with insect juvenoid analog while virus infection, and further feeding of the insect until its death of virus infection. According to the said method, juvenoid analog ZR-515 (Methoprene) is used to treat beet armyworm larva to raise the death rate of beet armyworm caused by nuclear polyhedron virus infection, raise monocephalic virus content and total virus yield. The method of the present invention can raise virus yield for pesticide.

Description

technical field [0001] The invention relates to the application of insect juvenile hormone analogues in the propagation of insect baculoviruses, in particular to a method for increasing the yield of insect baculoviruses by using insect juvenile hormone analogues. Background technique [0002] Insect baculovirus is an important pathogen of insects. At present, at least 600 kinds of insects (mainly Lepidoptera) have been found to be infected by baculovirus, and often cause insect epidemics in the wild. It is an important factor for regulating insect population density. pathogenic factors. Insect baculoviruses are highly specific to hosts. Generally, a virus can only infect one or several closely related hosts. It is safe and harmless to humans, livestock, fish, shrimp, and natural enemies, and does not pollute the environment. As early as the early 1970s, insect baculovirus was developed into a safe biopesticide and used in pest control, and ...

Claims

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Application Information

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IPC IPC(8): A61K38/22C12N7/01
Inventor 李广宏庞义陈其津杨凯
Owner SUN YAT SEN UNIV
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