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Cell 'pungent edge inhibition effect' and contact inhibition observation dishware and its application

A technology of inhibitory effect and contact inhibition, which is applied in tissue cell/virus culture devices, biochemical equipment and methods, and biochemical instruments to achieve objective radioactive contamination and no radioactive contamination.

Inactive Publication Date: 2002-06-19
SHANDONG TUMOR HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned method is a method for detecting the state of cell proliferation, and neither of them is a method for directly studying the regulation mechanism of cell proliferation; and the use of a detection device, especially a special vessel, to study the contact inhibition of cell growth has not been reported so far.

Method used

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  • Cell 'pungent edge inhibition effect' and contact inhibition observation dishware and its application
  • Cell 'pungent edge inhibition effect' and contact inhibition observation dishware and its application
  • Cell 'pungent edge inhibition effect' and contact inhibition observation dishware and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1 figure 1 The shown cell "sharp edge inhibition effect" and the bottom surface of the contact inhibition observation dish 3 are composed of a 35mm culture dish, a centrally located polystyrene cell culture observation plate 1 and its fixed steel clip 4; Mirror-polished stainless steel cylinder with a diameter of 0.5 mm is a cavity 2 that is ironed. The angle between the bottom surface of the cavity and its edge wall is 90 degrees. superior; figure 2 A conical hollow pouring head 7 is vertically erected in the center of the recess 2 shown, and the upper end of the pouring head is covered with a circular petal-shaped fixed disk 6, and the fixed disk is fixed to the middle of the dish body through a circular steel clip; image 3 There are three circular adjustment holes 8 on the petal-shaped perfusion head fixing plate, which are used for adjusting the position of the fixing plate and filling the cell culture solution.

[0034] The method for applying the cell ...

Embodiment 2

[0036] Example 2 figure 1 The shown cell "sharp edge inhibition effect" and the bottom surface of the contact inhibition observation dish 3 are composed of a 35mm culture dish, a centrally located polystyrene cell culture observation plate 1 and its fixed steel clip 4; Mirror-polished stainless steel cylinder with a diameter of 0.5 mm is a cavity 2 that is ironed. The angle between the bottom surface of the cavity and its edge wall is 90 degrees. Above: there is a conical hollow perfusion head 7 standing vertically beside the recess 2, the upper end of the perfusion head is covered with a circular petal-shaped fixed disk 6, and the fixed disk is fixed in the middle of the dish body through a circular steel clip; image 3 There are three circular adjustment holes 8 on the petal-shaped perfusion head fixing plate, which are used for adjusting the position of the fixing plate and filling the cell culture solution.

[0037] The method for applying the cell "sharp edge inhibition ...

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Abstract

The present invention discloses a cell "pungent edge inhibition effect" and contact inhibition viewing dish and its application method. Said viewing dish is formed from culture dish and a cell culture viewing plate which possesses a recess in the centre of its bottom disk and is placed in the culture dish, a perfusion head vertically-positioned in the recess, fixing disk of perfusion head and a fixing steel holder. The application of said device can accurately can visually make observation of cell "pungent edge inhibition effect" and detection of cell contact inhbition. Said device is unique in structure, can utilize microscope to make immediate tracking observation of number of cells, form and position.

Description

(1) Technical field [0001] The invention relates to a device for detecting cell proliferation regulation, in particular to a cell "sharp edge inhibition effect" and cell contact inhibition observation dish and application thereof. (2) Background technology [0002] Cells are an important material basis for organisms in nature, and the growth and development of humans and animals all depend on the proliferation and differentiation of cells. In medicine, the occurrence of many diseases is related to the abnormal regulation of human cell proliferation, especially the malignant tumor known as "incurable disease" - cancer. [0003] Contact inhibition is a characteristic of normal cells. It refers to the normal growth of adherent cells in a certain culture environment, attached to the surface of the culture vessel for adherent growth. When the cells grow to a confluent state and a certain density, the cells A state in which growth and proliferation have ceased. [0004] Malignan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12Q1/00
Inventor 国前于金明廖湘鲁李敏王兴武魏玲郑燕
Owner SHANDONG TUMOR HOSPITAL
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