Method of whole manual culture for chinese caterpillar fungus
A technology of artificial cultivation of Cordyceps sinensis, which is applied in the biological field, can solve the problems such as the inability to obtain Cordyceps sinensis, and achieve the effect of overcoming long growth time, large individuals, and high success rate of infection
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Embodiment 1
[0042] The full artificial culture method of embodiment 1 Cordyceps sinensis of the present invention
[0043] 1.1 Collection of samples
[0044] Cordyceps samples were collected from alpine shrub meadows at an altitude of 3,500 to 5,000 meters in Ganzi Prefecture, Sichuan.
[0045] 1.2 Separation of strains
[0046]1.2.1 Basic medium for separation: potato, glucose, agar, water, plus peptone (referred to as PDA + peptone) and potato, glucose, agar, water, plus silkworm chrysalis juice.
[0047] Its weight proportion: potato 200g, glucose 20g, agar 20g, peptone 10g, water 1000ml.
[0048] 1.2.2 Separation method
[0049] Sclerotia (worm body) and sub-seat separation: Sclerotia and sub-seats are surface-sterilized with 0.1% mercury chloride solution, washed with sterile water, cut into small sections or thin slices, placed on a culture dish with medium, and kept at 8- Incubate at 18°C.
[0050] Separation of ascospores: take the head of the ascospore, disinfect the surface...
Embodiment 2
[0092] The quality control of the Cordyceps sinensis that embodiment 2 inventive method cultivates
[0093] Select natural Cordyceps sinensis for comparison with the full artificial cultivation of Cordyceps sinensis of the present invention, take amino acid as example: sample is hydrolyzed through 6NHCl, temperature 110 ℃, time 24hr, analyze with Hitachi 835-50 amino acid analyzer, the result is as follows:
[0094] Natural Cordyceps %
[0095] The amino acid composition of the Cordyceps sinensis cultivated by the method of the invention is slightly higher than that of natural Cordyceps sinensis.
[0096] Adenosine (C 10 h 13 N 5 o 4 ) active ingredients as an example to the determination of the whole artificial culture of Cordyceps sinensis ("Chinese Pharmacopoeia" stipulates the determination of adenosine (C 10 h 13 N 5 o 4 ) content shall not be less than 0.010%):
[0097]
[0098] Through the measurement, it shows that the adenosine active ingre...
Embodiment 3
[0099] Cordyceps sinensis pharmacodynamics test cultivated by the method of the present invention in embodiment 3
[0100] Get 300g of Cordyceps sinensis cultivated by the method of the present invention, directly powder it, pack into capsules, and make capsules, and each capsule contains 0.3g of Cordyceps sinensis medicinal material.
[0101] The beneficial effect of the present invention is proved by regulating immunity, improving sleep, improving the pharmacodynamics test example of sleep quality now.
[0102] According to the test: the fully artificially cultivated Cordyceps sinensis has a sedative effect on the central nervous system; it has a significant effect on the respiratory system to expand the bronchus, and can significantly enhance the effect of adrenaline, and it also has the effect of eliminating phlegm, relieving asthma and preventing emphysema.
[0103] For the cardiovascular system, it can reduce myocardial oxygen consumption, improve myocardial ischemia, an...
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