Active peptide feed additive and its preparation method and use
A feed additive and active peptide technology, which is applied in the field of active peptide feed additive and its preparation, can solve the problems of allergic reaction to antigens, high preparation cost, difficult to popularize and use, and insignificant efficacy.
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Embodiment 1
[0073] Embodiment 1, prepare active peptide feed additive L-1 (liquid formulation)
[0074] The Bacillus subtilis was inserted into the sterilized liquid medium, and cultured at 25° C. for 48 hours to obtain the seed liquid.
[0075] The liquid culture medium is a mixed solution prepared in the following proportions: 1.3g of peptone, 3g of glucose, 3g of molasses, 50ml of potato infusion, 10g of soluble starch, 10g of sucrose, 2g of beef extract, 3.5g of yeast extract powder, soybean meal powder (60 mesh) 1.5g, growth factor 1ml, water 1000ml; the growth factor contains MgSO 4 .7H 2 O 20.00mg / ml, CaCl 2 .2H 2 O 2.20mg / ml, ZnSO 4 .7H 2 O 0.81mg / ml, FeSO 4 .7H 2 O 0.44mg / ml, MnSO 4 .H 2 O 0.13mg / ml, CuSO 4 .5H 2 O 0.02 mg / ml mixture.
[0076] The fermentation substrate - soybean protein powder is crushed to 60 meshes and then put into the fermenter, water and carbon source - cornmeal and molasses are added in turn (weight ratio is 1:1), the weight of the added water ...
Embodiment 2
[0091] Embodiment 2, prepare active peptide feed additive L-2 (liquid formulation)
[0092] Inoculate Aspergillus niger into the sterilized liquid medium, and culture at 35° C. for 24 hours to obtain seed liquid.
[0093] The liquid culture medium is a mixed solution prepared in the following proportions: 2.5g of peptone, 5g of glucose, 5g of molasses, 100ml of potato infusion, 20g of soluble starch, 30g of sucrose, 3g of beef extract, 5g of yeast extract powder, 2g of soybean meal, Growth factor 2ml, water 1000ml; the growth factor contains MgSO 4 .7H 2 O 30.00mg / ml, CaCl 2 .2H 2 O3.50mg / ml, ZnSO 4 .7H 2 O 1.10mg / ml, FeSO 4 .7H 2 O 0.60mg / ml, MnSO 4 .H 2 O 0.20mg / ml, CuSO 4 .5H 2 O 0.04 mg / ml mixture.
[0094] Fermentation substrate-soybean meal is crushed to 120 meshes and then put into the fermenter, water and carbon source-corn molasses and white sugar (weight ratio=3:2) are added successively, the weight of the added water is the same as that of the fermentati...
Embodiment 3
[0095] Embodiment 3, prepare active peptide feed additive L-3 (liquid formulation)
[0096] Inoculating Saccharomonas viridans into the sterilized liquid medium and culturing at 27° C. for 36 hours to obtain seed liquid.
[0097] The liquid culture medium is a mixed solution prepared in the following proportions: peptone 1.8g, glucose 4g, molasses 4g, potato extract 70ml, soluble starch 15g, sucrose 20g, beef extract 2.5g, yeast extract powder 4g, soybean meal powder (80 mesh) 1.8g, growth factor 1.5ml, water 1000ml; the growth factor contains MgSO 4 .7H 2 O 25.00mg / ml, CaCl 2 .2H 2 O 3.00mg / ml, ZnSO 4 .7H 2 O 1.00mg / ml, FeSO 4 .7H 2 O 0.50mg / ml, MnSO 4 .H 2 O0.17mg / ml, CuSO 4 .5H 2 O 0.03mg / ml mixed solution.
[0098] Put the fermentation substrate—bean cake into 80 meshes and put it into the fermenter, add water and carbon source—starch in sequence, the weight ratio of the weight of the water added to the protein contained in the fermentation substrate is 100:10,...
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