Extraction method of chlorella growth factor (chlorella extract and CGF)

A growth factor and extraction method technology, applied in the field of high-tech biology, can solve the problems of low yield, destroy functional components, and high cost, and achieve the effects of high residue utilization, low temperature requirements, and cost-effectiveness.

Inactive Publication Date: 2004-09-01
INST OF SOIL & FERTILIZER JIANGSU PROV ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, only a few developed countries have mastered the extraction technology of chlorella growth factor (chlorella essence, CGF) at present, and it is still blank in China.
According to the applicant's knowledge, the method of water extraction or alkaline solution extraction is mainly used in foreign countries. During the extraction process, some functional components are destroyed, resulting in poor activity of functional components, low output rate, high cost, and ineffective use of residues as waste. question

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The chlorella growth factor (chlorella extract) of the present embodiment is extracted and the final finished product is chlorella extract dry powder, and its steps are as follows:

[0019] A. Warm bath——Put chlorella powder or chlorella mud in warm water at 50°C to 70°C for about 10 hours to expand and soften the cell wall of chlorella;

[0020] B. Wall breaking—add pectinase to the chlorella pulp after the warm bath, and control the temperature at 40°C to 50°C for about 12 hours to hydrolyze the cell wall of the chlorella to form wall-broken algae mud;

[0021] C. Enzymatic hydrolysis - adding nuclease and protease to the broken chlorella pulp, and controlling the temperature at 40°C to 50°C to hydrolyze it into a mixture of chlorella essence;

[0022] C'. Separation - use centrifugal equipment to centrifuge the hydrolysis mixture to obtain the liquid of chlorella growth factor (chlorella essence);

[0023] D. Utilization of residues—spray-dry the separated residues ...

Embodiment 2

[0027] The chlorella growth factor (chlorella essence) extraction final product of this embodiment is chlorella essence capsule or tablet, and its steps are as follows:

[0028] A. Warm bath - Soak chlorella powder or chlorella mud in warm water at 50°C to 70°C to expand and soften the cell wall of chlorella;

[0029] B broken wall - add pectinase and cellulase to the chlorella pulp after warm bath, the temperature is controlled at 40 ℃ ~ 50 ℃, so that the chlorella cell wall undergoes hydrolysis reaction, and the broken wall algae mud is formed;

[0030] C. Enzymatic hydrolysis - adding nuclease and protease to the broken chlorella pulp, and controlling the temperature at 40°C to 50°C to hydrolyze it into a mixture of chlorella essence;

[0031] C'. Separation - use centrifugal equipment to centrifuge the hydrolysis mixture to obtain the liquid of chlorella growth factor (chlorella essence);

[0032] D. Utilization of residues—spray-dry the separated residues and process the...

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Abstract

A method for extracting chlorella growth factor (chlorella extract) belongs to the field of biological technology and includes the steps of warm-water bath, breaking wall, enzymolysis, separation and residue utilization. This invention adopts the method of adding enzyme to make hydrolysis extract the chlorella extract so that its temp. requirement is low and its solution is neutral. This invention also adopts the process of breaking wall and fully hydrolyzes amino acids and nucleotide, etc. contained in cell.

Description

technical field [0001] The invention relates to a method for extracting raw materials of nutraceuticals, in particular to an extraction method for chlorella growth factor (also known as chlorella essence, English Chlorella Growth Factor, CGF for short), which belongs to the field of high-tech biotechnology. Background technique [0002] Chlorella is the fastest growing plant in the world. No animal or plant can grow 4 times every 20 hours like Chlorella. This is mainly due to the growth factors of chlorella (chlorella essence, CGF). The growth factor of Chlorella (Chlorella essence, CGF) is a unique cell active substance of Chlorella, in which the hydrolyzed amino acid content reaches 38.8%, including amino acids (there are 16 kinds of amino acids, 8 of which are essential amino acids for human body), nucleic acid , The content of free nucleotides is higher than 6.8%, polysaccharides 7.4%, short-linked polypeptides, proteins, vitamins, minerals, and some "mysterious ingred...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/02
Inventor 韩士群
Owner INST OF SOIL & FERTILIZER JIANGSU PROV ACAD OF AGRI SCI
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