DNA biosensor for rapidly detecting clostridium perfringens in meat products and detection method thereof

Abstract

The invention discloses a DNA (deoxyribonucleic acid) biosensor for rapidly detecting clostridium perfringens in meat products and a detection method of the DNA biosensor. The biosensor comprises nano magnetic beads, DNA walker, DNA Aptamer, DNA H1, DNA H2, DNA Trigger, DNA H3 and DNA H4, wherein carboxyl groups are modified on the surfaces of the nano magnetic beads, and the nucleotide sequences of the DNA walker, the DNA Aptamer, the DNA H1, the DNA H2, the DNA Trigger, the DNA H3 and the DNA H4 are respectively shown as SEQ ID NO: 1-7. By introducing the magnetic nanoparticles and orderly assembling the nucleic acid aptamers on the surfaces of the magnetic nanoparticles, the operation steps in the detection process of the clostridium perfringens are greatly simplified, and the detection cost of the clostridium perfringens is reduced. Meanwhile, the detection method provided by the invention has the advantages of high sensitivity, low cost, rapidness and the like.

Description

technical field [0001] The invention relates to the field of detection, in particular to a DNA biosensor for rapidly detecting Clostridium perfringens in meat products and a detection method thereof. Background technique [0002] Clostridium perfringens is a Gram-positive anaerobic bacterium widely distributed in the environment, C. perfringens can grow in a wide temperature range of 5-50 °C, and 37-45 °C is its optimum temperature. At high growth temperatures, spores can survive for several years under harsh environmental conditions. C. perfringens can produce toxins that can easily lead to food-borne illnesses, including diarrhea and severe abdominal pain, and is a zoonotic pathogen. At present, the methods of detecting Clostridium perfringens by various means such as microbiology, immunology and biomolecular science are gradually being developed at home and abroad. [0003] Traditional microbial detection methods require long-term anaerobic culture and complicated verif...

AI Technical Summary

Problems solved by technology

[0003] Traditional microbial detection methods require long-term anaerobic culture and complicated verification, and have disadvantages such as time-consuming and high detection limits; although immunological methods can achieve rapid detection through antigen-antibody binding reactions, they are prone to false positives; biomolecular detection methods Has high sensitivity, but its detection cost is high

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