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Application of mesenchymal stem cell exosome

A bone marrow mesenchymal and stem cell technology, applied in the fields of biology and medicine, to reduce hepatic ischemia-reperfusion injury, prevent hypoxic injury, and inhibit cell apoptosis

Pending Publication Date: 2022-07-15
GUILIN MEDICAL UNIVERSITY
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Problems solved by technology

Studies have shown that exosomes from mesenchymal stem cells can regulate apoptosis and reduce hepatic ischemia-reperfusion injury. The mechanism needs to be further studied

Method used

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  • Application of mesenchymal stem cell exosome
  • Application of mesenchymal stem cell exosome
  • Application of mesenchymal stem cell exosome

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Embodiment Construction

[0041] Hereinafter, the present invention will be further described with reference to the accompanying drawings and specific embodiments. It should be noted that, on the premise of no conflict, the embodiments or technical features described below can be arbitrarily combined to form new embodiments. . In the following examples, unless otherwise indicated, all reagents used are of analytical grade, and all reagents used can be obtained from commercial sources. The experimental methods for which specific conditions are not indicated in the text are usually based on conventional conditions, such as those described in the book "Molecular Cloning Experiment Guide" published by Science Press, edited by J. Sambrook et al. in 2002, or as suggested by the manufacturer. conditions of. Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equival...

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Abstract

The invention provides an application of a bone marrow mesenchymal stem cell exosome. After BMSCs-Exo is injected into HIRI Kunming mice, liver function impairment can be relieved, and transcriptome sequencing finds that a p53 signal channel participates in the process. The miR-25-3p is significantly down-regulated in an HIRI liver, while the BMSCs and the BMSCs-Exo both contain the miR-25-3p. After the miR-25-3p simulant is transferred, the expression quantity of the miR-25-3p is increased. Bioinformatics analysis and dual luciferase reporter gene experiments show that PTEN is a target gene of miR-25-3p. After the miR-25-3p simulant is transferred into an HIRI mouse and hypoxia / reoxygenation AML12 hepatocytes, the PTEN expression quantity is reduced, the p53 pathway is inhibited, the apoptosis is reduced, the cell activity is improved, and the liver injury is relieved. Therefore, the mechanism of the miR-25-3p for relieving the HIRI is clarified, and a new thought is provided for stem cell treatment of the HIRI.

Description

technical field [0001] The present invention relates to the fields of biology and medicine, in particular to the application of bone marrow mesenchymal stem cell exosomes. Background technique [0002] Hepatic ischemia-reperfusion injury (HIRI) refers to a pathological phenomenon in which the blood supply to the liver is blocked for a period of time during liver surgery, and the blood flow is restored. . HIRI is a common clinical problem in the management of liver trauma, hepatic lobectomy, and liver transplantation. It can lead to organ failure, increase the incidence of rejection, and have a great impact on the prognosis and survival of patients. So far, there is still a lack of clinically effective interventions. In the process of ischemia-reperfusion, cells are in a state of oxidative stress, endoplasmic reticulum stress and calcium overload, mitochondrial swelling and outer membrane rupture, cytochrome C release, thereby triggering apoptosis signaling pathway, leading...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K31/7105A61P9/10
CPCA61K35/28A61K31/7105A61P9/10
Inventor 邢雪琨李红男刘润生张佳阳亢小敏林柯庄王瑞华陈晓琳牙海妮江俞臻
Owner GUILIN MEDICAL UNIVERSITY
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