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Multi-cardinal-number regulation and control culture method based on sulfide bacteria

A cultivation method and sulfide technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve problems such as poor use effect, achieve good use effect, avoid low activity, and solve poor use effect. good effect

Pending Publication Date: 2022-06-28
肖尊平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a multi-radix control and culture method based on sulfide bacteria, which has the advantage of good use effect and solves the problem of poor use effect of the previous sulfide bacteria cultivation process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A multi-cardinality-regulated and sulfide-based culture method for bacteria, comprising the following steps:

[0031] Step a: the user sterilizes the instruments required for the culture, and the sterilized instruments are dried by the drying device;

[0032] Step b: selecting the culture solution material by the user, pouring the culture solution material into the mixer by the user, and efficiently stirring and mixing the culture solution by the mixer;

[0033] Step c: the user pours the bacterial culture solution into the petri dish, the user adds bacterial strains to the petri dish, and the user moves the petri dish to the incubator;

[0034] Step d: setting the parameters of the incubator by the user, setting the temperature value, humidity value and light value of the culture, and replacing the culture solution in the culture dish in units of time;

[0035] Step e: The user periodically takes out the strain sample in the petri dish, the user observes the strain sa...

Embodiment 2

[0039] In embodiment 1, add the following steps again:

[0040] In the setting of the culture temperature in step d, the culture temperature in different time periods is set according to the strain culture temperature curve, and the rise and fall time between the temperatures in different time periods is set according to the needs of the strain culture.

[0041] A multi-cardinality-regulated and sulfide-based culture method for bacteria, comprising the following steps:

[0042] Step a: the user sterilizes the instruments required for the culture, and the sterilized instruments are dried by the drying device;

[0043] Step b: selecting the culture solution material by the user, pouring the culture solution material into the mixer by the user, and efficiently stirring and mixing the culture solution by the mixer;

[0044] Step c: the user pours the bacterial culture solution into the petri dish, the user adds bacterial strains to the petri dish, and the user moves the petri dis...

Embodiment 3

[0050] In embodiment 2, add the following steps again:

[0051] In step d, the culture humidity is set. According to the strain culture humidity curve, the culture humidity in different time periods is set, and the rise and fall time between the humidity in different time periods is set according to the needs of the strain culture.

[0052] A method for culturing bacteria based on multi-cardinality regulation and sulfide bacteria, comprising the following steps:

[0053] Step a: the user sterilizes the instruments required for the culture, and the sterilized instruments are dried by the drying device;

[0054] Step b: selecting the culture solution material by the user, pouring the culture solution material into the mixer by the user, and efficiently stirring and mixing the culture solution by the mixer;

[0055] Step c: the user pours the bacterial culture solution into the petri dish, the user adds bacterial strains to the petri dish, and the user moves the petri dish to th...

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Abstract

The invention discloses a multi-cardinal-number regulation and control culture method based on sulfide bacteria, which comprises the following steps: step a, disinfecting instruments required by culture by a user, and drying the disinfected instruments by a drying device; step b, selecting a culture solution material by a user, pouring the culture solution material into a mixing machine by the user, and efficiently stirring and mixing the culture solution by the mixing machine; and c, pouring the bacterial culture solution into a culture dish by a user, adding a bacterial strain into the culture dish by the user, and moving the culture dish into an incubator by the user. The sulfide bacteria cultivation device achieves the purpose of good use effect, and has the advantages of high-precision temperature control, humidity control, illumination control and monitoring, thereby avoiding bacterial colony cultivation failure or low activity, improving the experience feeling of a user on a sulfide bacteria cultivation process, meeting the requirements of the current market, and being worthy of popularization and application. The problem that an existing sulfide bacterium cultivation process is poor in use effect is solved.

Description

technical field [0001] The invention relates to the technical field of sulfide bacteria cultivation, in particular to a multi-base regulation and sulfide bacteria-based cultivation method. Background technique [0002] Sulfur bacteria are aerobic chemo-autotrophic bacteria, and their growth and decline characteristics mainly determine the amount of substrate (reducing sulfide) required by the bacteria in the soil and the suitability for changes in the environmental conditions of the microdomain around the cells. The process is used to cultivate sulfide bacteria, but most of the previous sulfide bacteria cultivation processes are ineffective and do not have the advantages of high-precision temperature control, humidity control, light control and monitoring, resulting in colony cultivation failure or low activity. , which reduces the user's experience of the sulfide bacteria cultivation process, and cannot meet the needs of today's market. Due to the above problems, a multi-ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 肖尊平
Owner 肖尊平
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