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Device for classifying invasive glioma based on DNA methylation

A glioma and classification device technology, which is applied in the field of invasive glioma classification devices, can solve the problems of unsatisfactory classification of gliomas and achieve high accuracy

Pending Publication Date: 2022-05-27
XIAMEN SPACEGEN BIOTECH CO LTD
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Problems solved by technology

[0003] The existing classification effect of glioma is not ideal, so there is a need for a classification device for infiltrating glioma based on DNA methylation to improve the above problems

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  • Device for classifying invasive glioma based on DNA methylation

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Embodiment

[0031] Example: Detecting abnormal DNA methylation by the detection module: DNA methylation refers to the use of S-adenosylmethionine (SAM) as a methyl donor under the action of DNA methyltransferase (DNMT). , adding a methyl group to the 5th carbon atom of cytosine of CpG base pair, about 2 / 3 of glioma genes have abnormal methylation in the promoter region, especially in the promoter region of tumor suppressor genes CpG island hypermethylation, methylation-specific real-time quantitative PCR was used to quantitatively analyze the methylation of MGMT promoter in 28 normal brain tissues and 50 gliomas. It was found that there was a significant MGMT promoter in glioma. Hypermethylation, the methylation rate of 25 CpG island sites of MGMT gene in oligodendroglioma was 88%, and the methylation rate of MGMT in glioma was 44%, in 183 glioma patients. (45 cases had MGMT promoter hypermethylation and 138 cases had no abnormal MGMT promoter methylation). Postoperative radiotherapy was ...

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Abstract

The invention relates to the technical field of biomedicine, in particular to a DNA methylation-based invasive glioma classification device, which comprises a detection module, a classification module and an information display module, whether neuroglial cells have canceration or not and the canceration degree are predicted by measuring the methylation degree of a CpG island, neuroglioma is classified, and by adopting real-time quantitative methylation specific PCR, the sensitivity and the accuracy are high, the DNA methylation condition of formalin soaked specimen tissue can be detected, and the detection result is accurate. Therefore, the real-time quantitative methylation specific PCR has the potential of being used for glioma diagnosis, mi RNA can stably exist in serum and is easy to obtain and has the potential of becoming an early diagnosis molecular marker, and the content of mi R-25 and mi R-223 in the serum can be used as a diagnosis marker of a non-small cell lung cancer patient; a specific glioma mi RNA marker is screened out, DNA is extracted from blood of a patient, then the mi RNA expression level can be detected through PCR, and therefore early diagnosis can be conducted on glioma.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a classification device for invasive glioma based on DNA methylation. Background technique [0002] Epigenetics plays a crucial role in cancer research, and through DNA methylation, histone variants and noncoding RNAs exhibit extensive reprogramming properties. DNA methylation is a stable feature that reflects inter- and intra-tumor heterogeneity and has been used to classify different types of tumors. [0003] The existing classification of gliomas is not satisfactory, so a DNA methylation-based classification device for invasive gliomas is needed to improve the above problems. SUMMARY OF THE INVENTION [0004] The purpose of the present invention is to provide a classification device for invasive glioma based on DNA methylation, so as to solve the problems raised in the above background art. [0005] To achieve the above object, the present invention provides the follow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6851G16B40/20
CPCC12Q1/6886C12Q1/6851G16B40/20C12Q2600/154C12Q2600/178C12Q2531/113C12Q2561/113
Inventor 陈琰郭飞飞康灿昆陈志宏
Owner XIAMEN SPACEGEN BIOTECH CO LTD
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