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SNP (Single Nucleotide Polymorphism) marker closely linked with eggplant green fruit color Gv1 gene and application

A technology of fruit color and eggplant, applied in the field of molecular biology, can solve the problems of plant oxidative damage, accumulation of intermediate products, vitality change, etc., and achieve the effect of accelerating the improvement process

Active Publication Date: 2022-05-24
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

A problem with any gene in this process may lead to changes in the activity of the enzyme, resulting in the accumulation of excessive intermediate products, resulting in oxidative damage to the plant, and even cell death, thereby triggering the production of different chlorotic mutants
At present, the research on the gene mapping and the development of related molecular markers has not been carried out on the green fruit color Gv gene of eggplant

Method used

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  • SNP (Single Nucleotide Polymorphism) marker closely linked with eggplant green fruit color Gv1 gene and application
  • SNP (Single Nucleotide Polymorphism) marker closely linked with eggplant green fruit color Gv1 gene and application
  • SNP (Single Nucleotide Polymorphism) marker closely linked with eggplant green fruit color Gv1 gene and application

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Experimental program
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Effect test

Embodiment 1

[0030] Example 1 Construction and Genetic Analysis of Genetic Population

[0031] 1. Test materials

[0032] Plant material: One purified inbred line 19141 of eggplant with white fruit color, which was bred by several generations of strict bagging self-breeding in the Vegetable Research Institute of Guangdong Academy of Agricultural Sciences, was used as the female parent (genotype: gv1gv1) and one purified self-cultivation with green fruit color. The inbred line 19143 is the male parent (genotype is Gv1Gv1), and the hybrid combination 20183F is prepared 1 , F 1 20183F was obtained by selfing a single plant 2 . Female parent, male parent and their F 1 During the whole growth period, the synthesis of anthocyanins was not observed in hypocotyls, leaf veins, flowers, and sepals; F 2 The segregation ratio of green fruit color and white fruit color plants in the segregation generation was in line with 3:1 by chi-square test ( figure 1 ).

[0033] 2. Genetic analysis

[003...

Embodiment 2

[0035] Embodiment 2 Eggplant fruit color epistatic gene location and SNP marker development

[0036] 1. Parent and 20183F 2 DNA preparation of green and white pools

[0037] 10 seedling leaves were taken from the male and female parents respectively, and the leaf DNA was extracted respectively, and the same amount of DNA was mixed from each plant to form the male parent pool and the female parent pool; F 2 The colony was isolated, the leaves were taken from each plant at the seedling stage, DNA was extracted separately, and the fruit color was investigated at the fruit setting stage. The DNA of 30 green fruit-colored plants was mixed in equal amounts to construct a green fruit-color pool (R20183G bulk), and 30 white-fruit-colored single plants were taken. The DNA of the strains was mixed in equal amounts to construct a ginkgo-colored pool (R20183Wbulk).

[0038] 2. Super-BSA technology to localize the eggplant green fruit color Gv1 gene

[0039] Beijing Baimaike Biotechnolo...

Embodiment 3

[0053] The detection application of embodiment 3 SNP 80595470 molecular marker in eggplant natural population

[0054] The SNP 80595470 molecular marker site was detected in 66 eggplant natural populations (inbred lines) using the SBJ80.59 primer. The results showed that 2 materials (A03 and A26) in the 15 ginkgo color mutant eggplants had HEX signals, indicating that their The mutation site is the same as that of the ginkgo parent 19141; among the 51 materials with green pericarp color, except 4 materials whose signals cannot be judged, the remaining 47 materials are all FAM signals (Table 2). The above results indicated that the ginkgo color phenotype on eggplant was caused by mutation of different chlorophyll metabolic pathways, and the whitening of eggplant fruit color caused by gv1gv1 mutation was only one of them. Further analysis of the fluorescence signal could not determine the source of the material. It was found that 21256 and 21261 were of the red eggplant type, th...

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Abstract

The invention belongs to the field of molecular biology, and relates to positioning of an eggplant green fruit color gene and development of a molecular marker, in particular to an SNP marker closely linked with an eggplant green fruit color Gv1 gene and application of the SNP marker. The method comprises the following steps: firstly, preliminarily positioning the Gv1 gene in a relatively small associated region between 757063683 and 83368320 on an eggplant No.8 chromosome; then, an SNP molecular marker SNP 80595470 closely linked with the eggplant green fruit color Gv1 gene is obtained in the associated region; finally, the genotypes of segregation population single plants under the specific genetic background can be accurately distinguished by utilizing specific primers of the molecular marker based on the PARM technology. The molecular marker and the molecular marker amplification primer can be applied to eggplant fruit color breeding practice in a simple, convenient, rapid and high-throughput mode, the improvement process of eggplant fruit color characters is accelerated, and meanwhile a good foundation is laid for cloning of the eggplant green fruit color Gv1 gene and functional research of the eggplant green fruit color Gv1 gene.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to the location of eggplant green fruit color gene and the development of molecular markers, in particular to the SNP marker closely linked with the eggplant green fruit color Gv1 gene and its application. Background technique [0002] Eggplant (Solanum melongena L.) belongs to the genus Solanum (Solanum L.) of the family Solanaceae and occupies an important position in the production of vegetables in the family Solanaceae. Eggplant is one of the top ten healthy vegetables in the world recommended by WHO. Eggplant peel color (hereinafter collectively referred to as fruit color) is rich and diverse, including purple-black, purple-red, green, white, pink, etc. Since different regions have different fruit color selection habits, fruit color is an important breeding target in the process of eggplant breeding. . Eggplant fruit color inheritance is a complex trait controlled by multiple ge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2600/172C12Q2531/113C12Q2563/107Y02A50/30
Inventor 孙保娟吕紫见李植良李涛宫超衡周游倩
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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