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Cross pollination by liquid-mediated delivery of pollen onto closed stigmas of flowers from recipient plants

A pollen and plant technology, applied in the field of agricultural biology, can solve time-consuming, labor-intensive, complex and other problems

Pending Publication Date: 2022-05-13
MONSANTO TECH LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the anatomy and size of the flower, this process is complex, time-consuming and labor-intensive (Walker et al., 1979; Talukdar et al., 2012)

Method used

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  • Cross pollination by liquid-mediated delivery of pollen onto closed stigmas of flowers from recipient plants
  • Cross pollination by liquid-mediated delivery of pollen onto closed stigmas of flowers from recipient plants
  • Cross pollination by liquid-mediated delivery of pollen onto closed stigmas of flowers from recipient plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Improved method for pollen collection, purification and storage

[0040] In some plants, especially soybeans, pollen collection is difficult due to the size and structure of the flowers. Furthermore, soybean pollen grains are relatively small in size (approximately 26 μm) and are present in relatively small quantities in a single flower (approximately 3000 to 7000 pollen grains / flower) (Palmer et al., 1978). To meet the needs of large-scale pollination using the methods described here, liquid-based platforms can be developed to optimize collection, purification, and storage of donor pollen. This includes the steps of breaking up the collected flowers (e.g., by grinding or homogenizing by using a bead mill homogenizer with or without liquid) to release the pollen, and filtering the broken flowers to purify the released pollen. pollen, the purified pollen was collected by centrifugation, and the purified pollen was resuspended into a solution suitable for stor...

Embodiment 2

[0042] Example 2. Development of solutions for pollen delivery

[0043] For liquid pollination of plants, pollen grains obtained from donor plants can be mixed into a liquid solution to facilitate delivery into flower buds. The components and their concentrations in the pollen liquid solution are important to the efficacy of the solution because they affect not only the pollen viability itself, but also the success of the pollinated plant in producing hybrid seed sets. However, while efficacy can be improved by optimizing the components and concentrations in a given pollen solution, many substitutions and modifications can be made while still achieving pollination, as shown in Table 1 below.

[0044] Table 1. Measurements of pod success when pollinated using various pollen solutions

[0045]

[0046] In order to reduce the surface tension between the pollen liquid solution and plant tissue, tests were performed to identify suitable surfactants that would not interfere with...

Embodiment 3

[0051] Example 3. Liquid-mediated pollen delivery in soybean plants

[0052] A suitable liquid-mediated pollen delivery method was assessed by the dye inspection method using soybean flower buds. Injection buffer (20% sucrose, 0.04% xanthan gum, 15U / LPME, 0.001% Tween 20, and Allura Red AC 0.01%) was injected by inserting a syringe needle at the bending point of the longest sepal until excess liquid seeped out. Injection buffer is delivered into hooded flower buds. Five minutes after injection, flower buds were dissected to check for red staining on the stigmas. Red staining on the stigma indicates that the applied liquid successfully contacted the stigma ( Figure 5 D).

[0053] Based on the results obtained from experiments testing the individual components, it was determined that beneficial components of solutions for pollen delivery in soybeans include, but are not limited to, the following components: Xanthan Gum: 0.04%-0.08% (w / v); Sucrose: 10-20% (w / v); PME: 0.01-10...

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PUM

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Abstract

The present invention provides a novel method of delivering pollen grains onto closed stigma in a recipient female flower by liquid mediation. For example, methods of liquid-mediated pollination are provided. The provided method comprises collecting pollen from a donor plant, suspending the collected pollen in a liquid solution, and introducing the solution onto a closed stigma of a recipient flower bud on a recipient plant, thereby pollinating a flower with pollen from the donor plant.

Description

technical field [0001] The present disclosure relates to the field of agricultural biotechnology, and more particularly to methods of improving cross-pollination efficiency by liquid-mediated delivery of donor plant pollen grains to closed stigmas of recipient plant flowers. [0002] Incorporation of Sequence Listings [0003] The sequence listing contained in the file named "MONS456WO_ST25.txt" (12 kilobytes in size as measured in the Microsoft Windows operating system, created on October 1, 2019) is hereby submitted electronically and adopted by Incorporated herein by reference. Background technique [0004] Hybridization is an important aspect of domesticated plant breeding because it enables the introduction of transient hybrid vigor, desired variation between different accessions, integration of transgenic traits, and generation of novel phenotypes (Goulet et al., 2017). Plant breeders use hybridization, or controlled cross-pollination, as the starting point for breed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/06A01H1/02A01H1/04A01H5/02A01H5/10A01H6/00A01H6/54C12N15/82
CPCA01H1/02A01H1/026A01H1/045C12N15/8201C12N15/8206C12N15/8213A01H1/023A01H6/542
Inventor H·W·拉鲁林莉
Owner MONSANTO TECH LLC
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