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Bioactive peptide prepared from snails as well as preparation method and application of bioactive peptide

A technology of bioactive peptides and snails, which is applied in the field of active peptides to achieve the effects of good flavor, light color, and improved taste and aroma

Active Publication Date: 2022-05-13
世联生物工程无锡有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most research reports on snails focus on the extraction of polysaccharides, helicase, and snail mucus. The bioactive peptide has safety, biocompatibility and excellent physiological activity, and will have broad application prospects

Method used

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  • Bioactive peptide prepared from snails as well as preparation method and application of bioactive peptide
  • Bioactive peptide prepared from snails as well as preparation method and application of bioactive peptide
  • Bioactive peptide prepared from snails as well as preparation method and application of bioactive peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] This embodiment provides a method for preparing biologically active peptides from snail protein, which specifically includes the following steps:

[0048] S1. Processing of snails: wash the snails, remove the shells, and separate the viscera and snail meat;

[0049] S2. Preparation of helicase: add phosphate buffer solution with a pH value of 5.5 to the viscera obtained in step S1, the mass volume ratio of the viscera to buffer is 1:3g / mL, pulverize with a meat grinder, and centrifuge at 3000r / min for 15min , taking the supernatant, using an ultrafiltration membrane with a pore size of 1000D to concentrate to a relative water density of 2, and freeze-drying to obtain helicase;

[0050] S3. Preparation of minced snail meat: crushing the snail meat with a meat grinder to obtain minced snail meat;

[0051] S4. Snail enzyme enzymolysis: Add 75 parts by weight of water and 2 parts by weight of helicase obtained in step S2 to 50 parts by weight of the minced snail meat obtai...

Embodiment 2

[0059] This embodiment provides a method for preparing biologically active peptides from snail protein, which specifically includes the following steps:

[0060] S1. Processing of snails: wash the snails, remove the shells, and separate the viscera and snail meat;

[0061] S2. Preparation of helicase: Add phosphate buffer solution with a pH value of 6 to the viscera obtained in step S1, the mass volume ratio of the viscera to the buffer solution is 1:5g / mL, pulverize with a meat grinder, and centrifuge at 3000r / min for 15min , taking the supernatant, using an ultrafiltration membrane with a pore size of 2000D to concentrate to a relative water density of 4, and freeze-drying to obtain helicase;

[0062] S3. Preparation of minced snail meat: crushing the snail meat with a meat grinder to obtain minced snail meat;

[0063] S4. Helicase enzymolysis: Add 100 parts by weight of water and 5 parts by weight of helicase prepared in step S2 to 50 parts by weight of the minced snail me...

Embodiment 3

[0071] This embodiment provides a method for preparing biologically active peptides from snail protein, which specifically includes the following steps:

[0072] S1. Processing of snails: wash the snails, remove the shells, and separate the viscera and snail meat;

[0073] S2. Preparation of helicase: add phosphate buffer solution with a pH value of 5.7 to the viscera obtained in step S1, the mass volume ratio of the viscera to the buffer solution is 1:4g / mL, pulverize with a meat grinder, and centrifuge at 3000r / min for 15min , taking the supernatant, using an ultrafiltration membrane with a pore size of 1500D to concentrate to a relative water density of 3, and freeze-drying to obtain helicase;

[0074] S3. Preparation of minced snail meat: crushing the snail meat with a meat grinder to obtain minced snail meat;

[0075] S4. Helicase enzymolysis: Add 85 parts by weight of water and 3.5 parts by weight of helicase prepared in step S2 to 50 parts by weight of the minced snail...

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Abstract

The invention provides a bioactive peptide prepared from snails as well as a preparation method and application of the bioactive peptide, and belongs to the technical field of bioactive peptides. Comprising the following steps: S1, treating snails; s2, preparation of helicase; s3, preparing minced snail meat; s4, performing enzymolysis by using helicase; s5, performing color protection treatment; s6, compound enzyme enzymolysis; s7, preparing a culture medium; s8, activating the strain; s9, performing fermentation; and S10, complexing. The prepared bioactive peptide can promote in-vivo environment balance, improve the immune function of the body, improve oxidative stress, improve anti-aging activity and inhibit generation of inflammatory factors, can also achieve an obvious anti-tumor effect, has excellent physiological activity and has a wide application prospect.

Description

technical field [0001] The invention relates to the technical field of active peptides, in particular to a bioactive peptide prepared from snails and its preparation method and application. Background technique [0002] Snails belong to gastropod terrestrial molluscs, which have high edible and medicinal value. The species commonly bred in China is the white jade snail, whose mucus mainly contains active ingredients such as collagen, allantoin, chondroitin sulfate, vitamins, and natural antimicrobial peptides. Among them, allantoin can regenerate the skin; protein and vitamins can increase the nutrition of the skin, making the skin more delicate and smooth. At present, most of the application and development of snail products are concentrated in the fields of food and cosmetics, and the research on it is mainly the extraction of polysaccharides, helicase, and secretions, but there is no research report on the extraction of cosmetic-grade snail mucus. [0003] "Comparative ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C12P21/00A61K38/01A61P39/06A61P35/00C12R1/23C12R1/01
CPCC12P21/06C12P21/00A61K38/012A61P39/06A61P35/00Y02A50/30
Inventor 陈和平陈敏
Owner 世联生物工程无锡有限公司
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