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Nano-enzyme compounded by copper nano-particles and ferriporphyrin nano-sheets as well as preparation and application of nano-enzyme

A technology of nanoparticles and nanosheets, applied in the field of imitation enzyme catalysis, can solve the problems of increasing the cost of imitation enzymes, and achieve the effect of enhancing root peroxidase activity and efficient decolorization

Pending Publication Date: 2022-05-13
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these technologies are based on the growth of noble metal materials on two-dimensional MOFs materials to form composite materials to improve their catalytic activity, which increases the cost of simulated enzymes.

Method used

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  • Nano-enzyme compounded by copper nano-particles and ferriporphyrin nano-sheets as well as preparation and application of nano-enzyme
  • Nano-enzyme compounded by copper nano-particles and ferriporphyrin nano-sheets as well as preparation and application of nano-enzyme
  • Nano-enzyme compounded by copper nano-particles and ferriporphyrin nano-sheets as well as preparation and application of nano-enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Preparation of nanozymes composited with copper-based nanoparticles and iron porphyrin nanosheets:

[0043] (1) The Cu-FeTCPP nanosheet prepared in Comparative Example 1 is dispersed in water so that its concentration is 0.3 mg / mL;

[0044] (2) Take 10 mL of the solution in step (1), add 0.1 mL of an aqueous solution of copper chloride with a concentration of 0.12 M, and mix well, then add dropwise 1 mL of an aqueous ammonia solution with a mass fraction of 0.1%, and react at 20° C. for 1 min;

[0045] (3) The product is centrifuged and precipitated and washed with pure water for 3 times to obtain the nanozyme.

[0046] The main components of copper-based nanoparticles include copper hydroxide and copper oxide, such as figure 2 As shown, the copper-based nanoparticles are in the form of nanoparticles, nanoneedles and nanorods, among which nanoparticles and nanoneedles are the main ones. 300nm, uniformly distributed on the surface of the nano-sheets; the mass ratio of...

Embodiment 2

[0059] Preparation of nanozymes composited with copper-based nanoparticles and iron porphyrin nanosheets:

[0060] (1) The Cu-FeTCPP nanosheet prepared in Comparative Example 1 is dispersed in water so that its concentration is 0.3 mg / mL;

[0061] (2) Take 10 mL of the solution in step (1), add 0.15 mL of an aqueous solution of copper chloride with a concentration of 0.12 M, and mix well, then add dropwise 1.2 mL of an aqueous ammonia solution with a mass fraction of 0.1%, and react at 25° C. for 3 h;

[0062] (3) The product is centrifuged and precipitated and washed with pure water for 3 times to obtain the nanozyme.

[0063] The main components of copper-based nanoparticles include copper hydroxide and copper oxide, such as Figure 5 As shown, the morphology of copper-based nanoparticles is mainly in the shape of nanoneedles and nanorods. The diameter of nanoneedles is 20-40 nm, and the length is 100-300 nm. The diameter of nano-rods is 50-70 nm, and the length is 200-300 ...

Embodiment 3

[0076] Preparation of nanozymes composited with copper-based nanoparticles and iron porphyrin nanosheets:

[0077] (1) The Cu-FeTCPP nanosheet prepared in Comparative Example 1 is dispersed in water so that its concentration is 0.3 mg / mL;

[0078] (2) Take 10 mL of the solution in step (1), add 0.2 mL of an aqueous solution of copper chloride with a concentration of 0.12 M, and mix well, then add dropwise 1.5 mL of an aqueous ammonia solution with a mass fraction of 0.1%, and react at 25° C. for 6 h;

[0079] (3) The product is centrifuged and precipitated and washed with pure water for 3 times to obtain the nanozyme.

[0080] The main components of copper-based nanoparticles include copper hydroxide and copper oxide, such as Figure 6As shown, the morphology of copper-based nanoparticles is dominated by nanoneedles and nanorods. The diameter of the nanoneedles is 20-40 nm, and the length is 100-300 nm. The diameter of the nano-rods is 50-70 nm, and the length is 200-300 nm. ...

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Abstract

The invention provides a nano-enzyme compounded by copper nanoparticles and ferriporphyrin nanosheets and a preparation method thereof, and can solve the problem that in the prior art, preparation of a mimic enzyme composite nano-material is limited to precious metal. According to the technical scheme, the ferriporphyrin nano-enzyme comprises a composite nano-enzyme formed by in-situ growth of non-noble metal copper nano-particles on the surface of a ferriporphyrin nano-sheet, wherein the nano-enzyme has horseradish peroxidase-like activity. The prepared nano-enzyme is used for realizing degradation of Congo red dye and detection of glucose. The method can be applied to the aspects of mimic enzyme catalysis and quantitative analysis in the technical fields of chemical industry, environment and biology.

Description

technical field [0001] The invention belongs to the field of imitation enzyme catalysis, and in particular relates to a nanozyme composed of copper-based nanoparticles and iron porphyrin nanosheets, a preparation method and application thereof. Background technique [0002] Natural enzymes have high catalytic efficiency and mild reaction conditions, but the stability of natural enzymes is poor, easy to inactivate, difficult to store, and high cost, which limits their application. Therefore, finding a material with both enzymatic activity and high stability has become a research hotspot. Nanozyme is a kind of nanomaterial with simulated enzyme activity. The discovery and research of nanozyme fills the shortcomings of natural enzymes. Among the existing materials that mimic enzyme activity, iron porphyrin metal-organic frameworks (MOFs), especially iron porphyrin MOFs nanosheets with a two-dimensional structure, are a promising enzyme-mimicking catalyst (CN202010342037.8). ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J31/22B01J31/18B01J23/72C02F1/72G01N21/33
CPCB01J31/183B01J31/1691B01J23/72C02F1/722G01N21/33B01J2531/16B01J2531/842B01J2531/025B01J2531/0213C02F2101/308B01J35/393B01J35/23B01J35/33
Inventor 赵莉芝张玉忠佀迪迪王立宪李日舜
Owner TIANJIN POLYTECHNIC UNIV
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