Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

New strain of methylobacterium, composition comprising same and use thereof as biostimulant and endophytic nitrogen-fixing bacteria

A Methylobacillus and composition technology, applied in the field of agronomy, can solve the problems such as the inability to replace chemical fertilizers, the inoculants that cannot achieve satisfactory results, the reduction of plant nitrogen units, and the like

Active Publication Date: 2022-04-29
西姆博格有限公司
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the known inoculants do not give satisfactory results in terms of stimulating plant growth, nor do they replace chemical fertilizers, nor do they significantly reduce the use of such chemical products
[0008] Most of the microorganisms known to be used as growth stimulants and / or nitrogen fixers cannot produce significant nitrogen content to significantly reduce the nitrogen units required for plant growth

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • New strain of methylobacterium, composition comprising same and use thereof as biostimulant and endophytic nitrogen-fixing bacteria
  • New strain of methylobacterium, composition comprising same and use thereof as biostimulant and endophytic nitrogen-fixing bacteria
  • New strain of methylobacterium, composition comprising same and use thereof as biostimulant and endophytic nitrogen-fixing bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Test 1. Routes of Administration of the Strains of the Invention

[0100] Experiments were carried out in a cultivation room where the strain of the present invention, namely the Novel Methylobacterium sp. strain with the deposit number CECT 9580 was applied by foliar application, soil application and seed coating (ie seed dressing).

[0101] The presence of the strains of the invention was assessed in leaves, stems and roots. The travel time of the strain in the plant from root to leaf and from leaf to root, as well as the penetration of the strain through root stomata and seeds were also evaluated.

[0102] To demonstrate the entry of Methylobacterium into plants and the colonization of different tissues, an experiment was performed in which seeds were inoculated into small litters and maize plants were inoculated into culture pots. In this case, three treatments were used: seed coating, application by irrigation and foliar application. Roots, stems and leaves of th...

Embodiment 3

[0193] Test 3. Efficacy of reducing nitrogen fertilizer by 30% in short-cycle planting of maize using the novel Methylobacterium sp. strain of the present invention.

[0194] ·Purpose

[0195] Methylobacteriaceae of the present invention are understood by green evolution of leaves (SPAD), vegetation index, nitrogen levels in plants, concentration of microorganisms in leaves and production under conventional growth conditions with 30% reduced nitrogen cover fertilization Efficacy of a new strain of the genus on maize (maize) development.

[0196] ·Materials and methods

[0197] Test fields and fertilization

[0198] The trial was carried out in Spain in two fields: one in Pomar de Cinca, Huesca, at coordinates 41°50'13.8"N, 0°05'38.0"E; the other in Finca Orán, Albacete, at coordinates 38° 49'55.3"N, 1°50'27.1"W.

[0199] Fertilization recommendations are regular fertilization and a 30% nitrogen reduction treatment. For this purpose, a fertilizer applicator was used ...

Embodiment 4

[0252] Experiment 4: Evaluation of the efficacy of the novel Methylobacterium sp. strain of the present invention in strawberry cultivation under different doses of nitrogen fertilization.

[0253] ·Purpose

[0254] The efficacy of the novel Methylobacterium spp. strains of the present invention on the development of strawberry crops fertilized with nitrogen at different doses was investigated by green evolution of leaves (SPAD), nitrogen levels in plants, concentration of microorganisms in leaves and yield, so The doses mentioned are 0, 25, 50, 75 and 100% of conventional fertilization.

[0255] ·Materials and methods

[0256]

[0257] Test fields and fertilization

[0258] The trials were carried out in the fields of the experimental farm in Symborg, Los Martínez del Puerto, Murcia, at coordinates 37°48'59.6"N, 1°05'43.6"W. The field area is 192m 2 , 8m wide and 24m long.

[0259] Irrigation and fertilization management was different for all treatments. The mi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of agronomy. In particular, the present invention relates to a strain of a new species of Methylobacterium sp. Nov. Having a preservation number of CECT 9580, a composition comprising the strain, and the use thereof as a biostimulant and an endophytic nitrogen-fixing bacterium in plants.

Description

[0001] field of invention [0002] The present invention relates to the field of agronomy. In particular, the present invention relates to Methylobacterium sp. nov. strains, compositions comprising the strains, and their use as biostimulants and endogenous nitrogen-fixing bacteria in plants. Background technique [0003] Many fertilizers are known in the field of agriculture. In particular, nitrogen-based fertilizers such as ammonia, ammonium nitrate (NH 4 NO 3 ) and urea, commonly used to keep crop yields as high as possible. In fact, nitrogen is involved in many biological processes in plants; for example, nitrogen is involved in the production of amino acids and thus proteins. [0004] However, only a small fraction of nitrogen-based fertilizers applied to crops is converted to plant matter. The amount of fertilizer that is not converted to plant matter may accumulate in the soil, or may run off into surface or groundwater, causing water pollution. [0005] In order t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/20A01N25/00A01N25/04A01N25/08A01P21/00C05G3/00C12N1/32C12N1/20C05F11/08C12R1/01
CPCC12N1/205C12N1/20A01N63/20A01P21/00C05F11/08C05G3/00C12R2001/01C12N1/32Y02W30/40Y02P60/21Y02E50/30A01N25/00A01N25/04A01N25/08A01N63/30
Inventor J·华雷斯莫利纳F·费尔南德斯马丁A·J·伯纳贝加西亚A·维拉马丁内兹F·J·莫雷诺穆诺兹R·托雷斯维拉
Owner 西姆博格有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com