Functionalized self-assembly miRNA/polypeptide composite hydrogel suitable for cartilage regeneration and preparation method thereof
A composite hydrogel and self-assembly technology, applied in the field of biomedical materials, can solve the problems of low cost performance and poor safety
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[0048] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The operating methods not indicated in the following examples are generally in accordance with conventional conditions, or in accordance with the conditions suggested by the manufacturer.
[0049] The functionalized self-assembled miRNA / polypeptide composite hydrogel suitable for cartilage regeneration in this example uses mouse miR-29b-5p and self-assembled polypeptide link recruitment factors (SKP, SKPPGTSS, amino acid sequence as shown in SEQ ID NO: 5 Shown) fragment, its preparation method comprises steps:
[0050] 1. Dissolve self-assembled polypeptide link recruitment factors (SKP, SKPPGTSS) in pure water, and obtain four sets of functional polypeptide solutions 1 after ultrasonic d...
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