Method for simultaneously detecting four mycotoxins based on multi-channel label-free electrochemical aptamer sensor
A technology of aptamer sensor and mycotoxin, which is applied in the field of electrochemical analysis, achieves the effects of low detection cost, simple preparation process and high specificity
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[0045] (1) Preparation and pretreatment of multi-channel ITO electrodes
[0046] Such as figure 1 As shown, cut the ITO conductive glass into a rectangle of 35×25mm, and then use laser etching to leave the required four working areas a, b, c, d (diameter = 6mm) and their respective conductive channels. Then the multi-channel ITO electrode was sonicated in acetone, ethanol and water for 10 min, respectively. Then, dry the multi-channel ITO electrode under an infrared lamp, and cover the surface of the ITO electrode with a brown adhesive tape with four round holes (25×25mm, the diameter of the round hole is 6mm, and the position coincides with the working area of the electrode).
[0047] (2) Four working areas to modify gold nanoparticles
[0048] In the three-electrode system, the reference electrode is a saturated calomel electrode, the counter electrode is a platinum wire electrode, and the working electrode is a multi-channel ITO electrode. in 2mM HAuCl 4 Electrodeposi...
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